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Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-HKDC1 antibody. Counter stained with hematoxylin.)

Rabbit HKDC1 Polyclonal Antibody | anti-HKDC1 antibody

HKDC1 Antibody

Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Protein affinity purified
Synonyms
HKDC1; Polyclonal Antibody; HKDC1 Antibody; BC016235 antibody; Hexokinase domain containing 1 antibody; Hexokinase domain-containing protein 1 antibody; Hkdc1 antibody; HKDC1_HUMAN antibody; Putative hexokinase HKDC1 antibody; anti-HKDC1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Protein affinity purified
Form/Format
1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-HKDC1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Immunogen
Recombinant protein within human HKDC1 260-460aa.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-HKDC1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-HKDC1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HKDC1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HKDC1 antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining HKDC1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining HKDC1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining HKDC1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining HKDC1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining HKDC1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining HKDC1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of LOVO cells with HKDC1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of LOVO cells with HKDC1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Related Product Information for anti-HKDC1 antibody
As the recently identified fifth isoform of hexokinase, HKDC1 catalyzes the rate-limiting and first obligatory step of glucose metabolism, which is the ATP-dependent phosphorylation of glucose to G6P. Though its particular biological function remains unclear, HKDC1 has been suggested to play a more major role in glucose metabolism during pregnancy, as the mother would need to provide enough energy for both herself and the fetus. HKDC1 is ubiquitously expressed, with the highest levels of expression in pharynx, thymus, colon, esophagus, and eye tissue.
Product Categories/Family for anti-HKDC1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
82,682 Da
NCBI Official Full Name
putative hexokinase HKDC1
NCBI Official Synonym Full Names
hexokinase domain containing 1
NCBI Official Symbol
HKDC1
NCBI Protein Information
putative hexokinase HKDC1
UniProt Protein Name
Putative hexokinase HKDC1
Protein Family
UniProt Gene Name
HKDC1
UniProt Entry Name
HKDC1_HUMAN

Uniprot Description

HKDC1: Belongs to the hexokinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.1; Kinase, other

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: cytosol

Molecular Function: ATP binding; fructokinase activity; glucokinase activity; glucose binding; mannokinase activity

Biological Process: carbohydrate phosphorylation; cell glucose homeostasis; glucose 6-phosphate metabolic process; glycolysis; hexose metabolic process

Research Articles on HKDC1

Similar Products

Product Notes

The HKDC1 hkdc1 (Catalog #AAA9435410) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HKDC1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HKDC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the HKDC1 hkdc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HKDC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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