Western Blot (WB)
(The anti-HK3 Pab is used in Western blot to detect HK3 in HL-60 cell lysate.)
Rabbit anti-Human, mouse HK3 (Hexokinase III) Polyclonal Antibody | anti-HK3 antibody
HK3 (Hexokinase III) Antibody (C-term)
Western Blot (WB)
(The anti-HK3 Pab is used in Western blot to detect HK3 in HL-60 cell lysate.)
Western Blot (WB)
(The anti-HK3 Pab is used in Western blot to detect HK3 in HL-60 cell lysate.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Western Blot (WB)
(HK3 Antibody (R775) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the HK3 antibody detected the HK3 protein (arrow).)
Western Blot (WB)
(HK3 Antibody (R775) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the HK3 antibody detected the HK3 protein (arrow).)
Palma, F., et al., Mol. Cell. Biochem. 155(1):23-29 (1996).
Colosimo, A., et al., Cytogenet. Cell Genet. 74(3):187-188 (1996).
NCBI and Uniprot Product Information
NCBI Description
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. [provided by RefSeq, Apr 2009]
Uniprot Description
HK3: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).
Protein type: Transferase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Enzyme, cellular metabolism; Carbohydrate Metabolism - galactose; Kinase, other; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - starch and sucrose; EC 2.7.1.1
Chromosomal Location of Human Ortholog: 5q35.2
Cellular Component: protein complex; mitochondrion; cytosol
Molecular Function: hexokinase activity; enzyme binding; mannokinase activity; hormone binding; fructokinase activity; glucokinase activity; ATP binding
Biological Process: hexose metabolic process; glycolysis; hexose transport; glucose 6-phosphate metabolic process; carbohydrate metabolic process; carbohydrate phosphorylation; cell glucose homeostasis; pathogenesis; glucose transport; transmembrane transport
Research Articles on HK3
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Product Notes
The HK3 hk3 (Catalog #AAA9210110) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 760-789. The HK3 (Hexokinase III) Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HK3 (Hexokinase III) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the HK3 hk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HK3 (Hexokinase III), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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