Western Blot (WB)
(Western Blot analysis of Recombinant HIV-1 gp120 Protein (group P, strain RBF168) (His Tag) (with 30ng) using Anti-HIV-1 gp120 Protein (group P, strain RBF168) Polyclonal Antibody at dilution of 1:1000.)
Rabbit HIV-1 gp120 Polyclonal Antibody | anti-HIV-1 antibody
Anti-HIV-1 gp120 Protein (group P, strain RBF168) Polyclonal Antibody
Reactivity
HIV-1
Applications
Western Blot, ELISA
Purity
Antigen Affinity
Synonyms
HIV-1 gp120; Polyclonal Antibody; Anti-HIV-1 gp120 Protein (group P; strain RBF168) Polyclonal Antibody; gp120; anti-HIV-1 antibody
Host
Rabbit
Reactivity
HIV-1
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity
Form/Format
0.2um filtered solution in PBS
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-HIV-1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-1:5000
ELISA: 1:5000-1:10,000
ELISA: 1:5000-1:10,000
Immunogen
Recombinant HIV-1 gp120 Protein (group P, strain RBF168) (His Tag)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of Recombinant HIV-1 gp120 Protein (group P, strain RBF168) (His Tag) (with 30ng) using Anti-HIV-1 gp120 Protein (group P, strain RBF168) Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of Recombinant HIV-1 gp120 Protein (group P, strain RBF168) (His Tag) (with 30ng) using Anti-HIV-1 gp120 Protein (group P, strain RBF168) Polyclonal Antibody at dilution of 1:1000.)
Related Product Information for anti-HIV-1 antibody
The HIV-1 gp120 envelope protein, a glycoprotein that is part of the outer layer of the virus, which is an essential component in the multi-tiered viral entry process. It presents itself as viral membrane spikes consisting of 3 molecules of gp120 linked together and anchored to the membrane by gp41 protein. Gp120 is essential for viral infection as it facilitates HIV entry into the host cell and this is its best-known and most researched role in HIV infection. However, it is becoming increasingly evident that gp12 might also be facilitating viral persistence and continuing HIV infection by influencing the T cell immune response to the virus. The surface protein gp120 attaches the virus to the host lymphoid cell by binding to the primary receptor CD4. Gp120 binding to its receptor CD4 and co-receptor, CXCR4 or CCR5 is required for fusion of viral and cellular membranes. Several mechanisms might be involved in this process of which gp120 binding to the CD4 receptor of T cells is the best known and most important interaction as it facilitates viral entry into the CD4+ cells and their depletion, a hallmark of the HIV infection. Gp120 is shed from the viral membrane and accumulates in lymphoid tissues in significant amounts. Despite the overall genetic heterogeneity of the gp120 glycoprotein, the conserved CD4 binding site provides an attractive antiviral target. Interaction between gp120 and ITGA4/ITGB7 would allow the virus to enter GALT early in the infection, infecting and killing most of GALT's resting CD4+ T-cells. This T-cell depletion is believed to be the major insult to the host immune system leading to AIDS.
Product Categories/Family for anti-HIV-1 antibody
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Product Notes
The HIV-1 (Catalog #AAA2563790) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HIV-1 gp120 Protein (group P, strain RBF168) Polyclonal Antibody reacts with HIV-1 and may cross-react with other species as described in the data sheet. AAA Biotech's HIV-1 gp120 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-1:5000 ELISA: 1:5000-1:10,000. Researchers should empirically determine the suitability of the HIV-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIV-1 gp120, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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