Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K36 antibody.)
Rabbit Histone H3 Polyclonal Antibody | anti-H3 antibody
Histone H3 (DiMethyl-K36) Polyclonal Antibody
Gene Names
H3-4; H3t; H3.4; H3/g; H3FT; HIST3H3
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
Histone H3; Polyclonal Antibody; Histone H3 (DiMethyl-K36) Polyclonal Antibody; H3.4; H3/g; H3FT; H3t; anti-H3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Clonality
Polyclonal
Isotype
IgG
Specificity
Histone H3 (DiMethyl-K36) polyclonal antibody detects endogenous levels of Histone H3 protein only when Di-methylated at Lys36.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
136
Applicable Applications for anti-H3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation (ChIP)-seq
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
IP: 1:50-1:200
ChIP: 1:20-1:100
CHIPseq: 1:20-1:50
IHC: 1:50-1:200
IF: 1:50-1:200
IP: 1:50-1:200
ChIP: 1:20-1:100
CHIPseq: 1:20-1:50
Immunogen
A synthetic methylated peptide corresponding to residues surrounding K36 of human histone H3
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K36 antibody.)
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K36 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human lung using DiMethyl-Histone H3-K36 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human lung using DiMethyl-Histone H3-K36 antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K36 antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K36 antibody.)
Related Product Information for anti-H3 antibody
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
18kDa
NCBI Official Full Name
histone H3.1t
NCBI Official Synonym Full Names
H3.4 histone
NCBI Official Symbol
H3-4
NCBI Official Synonym Symbols
H3t; H3.4; H3/g; H3FT; HIST3H3
NCBI Protein Information
histone H3.1t
UniProt Protein Name
Histone H3.1t
Protein Family
UniProt Gene Name
HIST3H3
UniProt Synonym Gene Names
H3FT; H3/t; H3t
UniProt Entry Name
H31T_HUMAN
NCBI Description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
Research Articles on H3
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Product Notes
The H3 hist3h3 (Catalog #AAA3011066) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3 (DiMethyl-K36) Polyclonal Antibody reacts with Human, Mouse, Rat, Other (Wide Range) and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation (ChIP)-seq. WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200 IP: 1:50-1:200 ChIP: 1:20-1:100 CHIPseq: 1:20-1:50. Researchers should empirically determine the suitability of the H3 hist3h3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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