Rabbit anti-Human DDB1 Polyclonal Antibody | anti-DDB1 antibody

DDB1, ID (DNA Damage-binding Protein 1, DDB p127 Subunit, DNA Damage-binding Protein a, DDBa, Damage-specific DNA-binding Protein 1, HBV X-associated Protein 1, XAP-1, UV-damaged DNA-binding Factor, UV-damaged DNA-binding Protein 1, UV-DDB 1, XPE-binding

Cat. Num. AAA646980

• Gene Names: DDB1; XPE; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Serum; Serum
• Synonyms: DDB1; Polyclonal Antibody; ID (DNA Damage-binding Protein 1; DDB p127 Subunit; DNA Damage-binding Protein a; DDBa; Damage-specific DNA-binding Protein 1; HBV X-associated Protein 1; XAP-1; UV-damaged DNA-binding Factor; UV-damaged DNA-binding Protein 1; UV-DDB 1; XPE-binding; Anti -DDB1; anti-DDB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human DDB1.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid, 0.1% sodium azide.

• Applicable Applications for anti-DDB1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:2000-1:10,000; Western Blot: 1:500-1:1000; Immunohistochemistry (Formalin fixed paraffin embedded): 1:500
• Immunogen: Synthetic peptide corresponding to aa198-213 from the internal region of human DDB1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with DDB1 antibody at 1:500 followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-DDB1 antibody

DNA damage binding protein (DDB) is a heterodimer that functions in DNA repair, and is associated with the DNA repair deficiency disease xeroderma pigmentosum (XP). The damaged DNA binding function of DDB requires both DDB1 (p125, 127kD) and DDB2 (p48, 48kD) subunits. When the subunits of DDB are expressed individually, p48 localized in the nucleus and p125 localized in the cytoplasm. Co-expression of p125 with p48 resulted in an increased accumulation of p125 in the nucleus, indicating that p48 plays a critical role in the nuclear localization of p125.

Product Categories/Family for anti-DDB1 antibody

Antibodies; Abs to DNA Replication; Repair

NCBI and Uniprot Product Information

• NCBI GI #: 148529014
• NCBI GeneID: 1642
• NCBI Accession #: NP_001914.3
• NCBI GenBank Nucleotide #: NM_001923.4
• UniProt Accession #: Q16531; O15176; Q13289; Q58F96; A6NG77; B2R648
• Molecular Weight: 126,968 Da
• NCBI Official Full Name: DNA damage-binding protein 1
• NCBI Official Synonym Full Names: damage-specific DNA binding protein 1, 127kDa
• NCBI Official Symbol: DDB1
• NCBI Official Synonym Symbols: XPE; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1
• NCBI Protein Information: DNA damage-binding protein 1; XAP-1; UV-DDB 1; DDB p127 subunit; XPE-binding factor; HBV X-associated protein 1; DNA damage-binding protein a; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; xeroderma pigmentosum group E-complementing protein
• UniProt Protein Name: DNA damage-binding protein 1
• Protein Family: DNA damage-binding protein
• UniProt Gene Name: DDB1
• UniProt Synonym Gene Names: XAP1; DDBa; XAP-1; UV-DDB 1; XPE-BF; XPCe
• UniProt Entry Name: DDB1_HUMAN

NCBI Description

The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins. [provided by RefSeq, May 2012]

Uniprot Description

Function: Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2. Ref.14 Ref.16 Ref.17 Ref.18 Ref.21 Ref.22 Ref.23 Ref.24 Ref.25 Ref.27 Ref.29 Ref.30 Ref.31 Ref.33 Ref.34 Ref.36

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Component of the UV-DDB complex which includes DDB1 and DDB2. The UV-DDB complex interacts with monoubiquitinated histone H2A and binds to XPC via the DDB2 subunit. Component of numerous DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which consist of a core of DDB1, CUL4A or CUL4B and RBX1. DDB1 may recruit specific substrate targeting subunits to the DCX complex. These substrate targeting subunits are generally known as DCAF (DDB1- and CUL4-associated factor) or CDW (CUL4-DDB1-associated WD40-repeat) proteins. Interacts with AMBRA1, ATG16L1, BTRC, DCAF1, DCAF17, DCAF16, DCAF15, DDA1, DET1, DTL, ERCC8, FBXW5, FBXW8, GRWD1, DCAF6, KATNB1, NLE1, NUP43, PAFAH1B1, PHIP, PWP1, RBBP4, RBBP5, RBBP7, RFWD2, SNRNP40, VPRBP, WDR5, WDR5B, WDR12, DCAF4, DCAF5, DCAF11, WDR26, DCAF10, WDR39, DCAF12, WDR42, DCAF8, WDR53, WDR59, WDR61, DCAF7, WSB1, WSB2, LRWD1 and WDTC1. DCX complexes may associate with the COP9 signalosome, and this inhibits the E3 ubiquitin-protein ligase activity of the complex. Interacts with NF2, TSC1 and TSC2. Interacts with Simian virus 5 protein V and the HBV X protein. Interaction with SV5 protein V may prevent the recruitment of DCAF proteins to DCX complexes. Interacts with AGO1 and AGO2. Associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and VPRBP proteins (EDVP complex). Interacts directly with DYRK2. DCX(DTL) complex interacts with FBXO11; does not ubiquitinate and degradate FBXO11. Ref.10 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.23 Ref.26 Ref.27 Ref.28 Ref.30 Ref.31 Ref.32 Ref.33 Ref.34 Ref.35 Ref.36 Ref.38 Ref.41 Ref.42 Ref.43 Ref.44 Ref.45

Subcellular location: Cytoplasm. Nucleus. Note: Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage. Ref.11 Ref.12 Ref.31 Ref.33

Post-translational modification: Phosphorylated by ABL1

By similarity.Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis. Ref.12

Sequence similarities: Belongs to the DDB1 family.