Rabbit Histone H3 Polyclonal Antibody | anti-H3 antibody

Polyclonal Antibody to Histone H3 (H3)

Cat. Num. AAA2025893

• Reactivity: Human, Mouse, Rat, Rabbit, Simian, Dog, Cattle, Horse, Gallus
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: Histone H3; Polyclonal Antibody; Polyclonal Antibody to Histone H3 (H3); anti-H3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Rabbit, Simian, Dog, Cattle, Horse, Gallus
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against H3. It has beenselected for its ability to recognize H3 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Liquid
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGSEF-MARTKQTARK STGGKAPRKQ LATKAARKSA PATGGVKKPH RYRPGTVALR EIRRYQKSTE LLIRKLPFQR LVREIAQDFK TDLRFQSSAV MALQEACEAY LVGLFEDTNL CAIHAKRVTI MPKDIQLARR IRGERA
• Sequence Length: 136

• Applicable Applications for anti-H3 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
• Application Notes: Western blotting: 0.5-2ug/mL; Immunohistochemistry: 5-20ug/mL; Immunocytochemistry: 5-20ug/mL; Optimal working dilutions must be determined by end user.
• Fragment: H3 (Met1~Ala136)
• Organism Species: Homo sapiens (Human),Mus musculus (Mouse),Rattus norvegicus (Rat),Cavia (Guinea pig),Oryctolagus cuniculus (Rabbit),Rhesus monkey (Simian),Bos taurus; Bovine (Cattle),Equus caballus; Equine (Horse),Chicken (Gallus)
• Cross Reactivity: Human
• Conjugate: No Conjugate
• Immunogen: Recombinant H3 (Met1~Ala136) expressed in E Coli.
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2040538
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Mouse Ovary Tissue; Lane2: Mouse Brain Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant H3, Mouse.)

Immunohistochemistry (IHC)

(FITC staining on IHC-P Simple: Rat Skeletal Muscle Tissue.)

Immunohistochemistry (IHC)

(FITC staining on IHC-P;Simple:Rat Spinal Cord Tissue)

Immunohistochemistry (IHC)

(FITC staining on IHC-P Simple: Rat Kidney Tissue)

Immunohistochemistry (IHC)

(FITC staining on IHC-P;Simple: Rat Adrenal Tissue)

Immunohistochemistry (IHC)

(FITC staining on IHC-P;Simple:Rat Placenta Tissue.)

Immunohistochemistry (IHC)

(FITC staining on IHC-P Simple: Rat Stomach Tissue.))

Immunohistochemistry (IHC)

(FITC staining on IHC-P Simple: Rat Small Intestine Tissue.)

Immunohistochemistry (IHC)

(FITC staining on IHC-P Simple: Rat Heart Tissue.)

Immunohistochemistry (IHC)

(FITC staining on IHC-P Simple:Rat Pancreas Tissue.)

Immunohistochemistry (IHC)

(HE staining on IHC-P; Samples: Rat Skin Tissue.)

Immunohistochemistry (IHC)

(HE staining on IHC-P; Samples: Rat Intestine Tissue.)

Immunohistochemistry (IHC)

(FITC staining on IHC-P; Simple: Rat Brain Tissue.)

(FITC staining on IHC-P Simple:Rat Testis Tissue)

NCBI and Uniprot Product Information

• NCBI GI #: 3273350
• Molecular Weight: 15,406 Da
• NCBI Official Full Name: histone H3
• UniProt Protein Name: Histone H3.3
• Protein Family: Histone
• UniProt Gene Name: H3
• UniProt Entry Name: H33_TOBAC

Uniprot Description

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.