Western Blot (WB)
(Western Blot Analysis: Representative blot from a previous lot. Recombinant Histone H3 (Lane 1) and HeLa acid extract (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed withMBS613333, (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates trimethyl-Histone H3 (Lys9) (~17kD).)
Rabbit Histone H3 Polyclonal Antibody | anti-HIST3H3 antibody
Histone H3, trimethyl (Lys9) (APC)
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human, C. elegans
Applications
Western Blot, Dot Blot, Immunofluorescence
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Histone H3; Polyclonal Antibody; trimethyl (Lys9) (APC); anti-HIST3H3 antibody
Host
Rabbit
Reactivity
Human, C. elegans
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human trimethyl-histone H3 (Lys9), Mr 17kD. Species Crossreactivity: C. elegans. Species sequence homology: mouse, rat and chicken. Broad species crossreactivity expected.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-HIST3H3 antibody
Western Blot (WB), Dot Blot (DB), Peptide Inhibition Assay, Immunofluorescence (IF)
Application Notes
WB: 1:500 of this lot detected trimethyl Histone H3 in acid extracted proteins from HeLa cells.
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
A synthetic peptide containing the 2X-branched sequence ...AR[me3K]ST... in which me3K corresponds to trimethyl-lysine at residue 9 of human histone H3 (KLH).
Conjugate
APC
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot Analysis: Representative blot from a previous lot. Recombinant Histone H3 (Lane 1) and HeLa acid extract (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed withMBS613333, (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates trimethyl-Histone H3 (Lys9) (~17kD).)
Western Blot (WB)
(Western Blot Analysis: Representative blot from a previous lot. Recombinant Histone H3 (Lane 1) and HeLa acid extract (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed withMBS613333, (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates trimethyl-Histone H3 (Lys9) (~17kD).)
Immunocytochemistry (ICC)
(Figure: ICC: Mouse embryonic fibroblasts were stained withMBS613333.)
Immunocytochemistry (ICC)
(Figure: ICC: Mouse embryonic fibroblasts were stained withMBS613333.)
Dot Blot (DB)
(Dot Blot Analysis: Representative data. A dilution series of linear histone H3 peptides containing the indicated modifications was made, spotted onto PVDF membranes and probed with a 1:1000 dilution ofMBS613333. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system)
Dot Blot (DB)
(Dot Blot Analysis: Representative data. A dilution series of linear histone H3 peptides containing the indicated modifications was made, spotted onto PVDF membranes and probed with a 1:1000 dilution ofMBS613333. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system)
Product Categories/Family for anti-HIST3H3 antibody
References
1. Kohlmaier, A., et al., PLoS Biol. 2: E171, 2004. 2. Perez-Burgos, L., et al., Meth. Enzymol. 376: 234-254, 2004. 3. Peters, A.H., et al., Mol Cell. 12: 1577-1589, 2003. 4. Rea, S., et al., Nature 406:593-599, 2000.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
136
NCBI Official Full Name
histone H3.1t
NCBI Official Synonym Full Names
histone cluster 3, H3
NCBI Official Symbol
HIST3H3
NCBI Official Synonym Symbols
H3t; H3.4; H3/g; H3FT
NCBI Protein Information
histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T
UniProt Protein Name
Histone H3.1t
Protein Family
UniProt Gene Name
HIST3H3
UniProt Synonym Gene Names
H3FT; H3/t; H3t
UniProt Entry Name
H31T_HUMAN
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Product Notes
The HIST3H3 hist3h3 (Catalog #AAA6480605) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3, trimethyl (Lys9) (APC) reacts with Human, C. elegans and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Dot Blot (DB), Peptide Inhibition Assay, Immunofluorescence (IF). WB: 1:500 of this lot detected trimethyl Histone H3 in acid extracted proteins from HeLa cells. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HIST3H3 hist3h3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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