Sheep anti-Bovine Histone H2B Polyclonal Antibody | anti-H2B antibody
Histone H2B
Gene Names
H2B; MGC159573
Reactivity
Bovine
Applications
Western Blot, Immunoprecipitation, Immunofluorescence
Purity
Affinity PurifiedPurified
Synonyms
Histone H2B; Polyclonal Antibody; Anti -Histone H2B; anti-H2B antibody
Host
Sheep
Reactivity
Bovine
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes bovine Histone H2b: 100%. No reaction with Histone-H-1, Histone-H-3 or Histone-H-4. Very low crossreactivity with Histone-H-core.
Purity/Purification
Affinity Purified
Purified
Purified
Form/Format
Supplied as a liquid in PBS. No preservatives added.
Applicable Applications for anti-H2B antibody
Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
Application Notes
Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence.
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:1000
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:1000
Immunogen
Histone 2B, calf whole protein complexed to ribosomal RNA.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-H2B antibody
Autoantibodies to histone antigens have been described in patients with idiopathic and drug-induced SLE, rheumatoid arthritis, and other conditions. The presence of autoantibodies to histones are frequently found in several rheumatic disorders. In one study, the predominant responses to histones in SLE sera were to H1, H2b, and H3. Marked elevations of binding occurred to H1 and H2b in 33% of patients, while 25% showed higher binding to H3. The same study showed the highest anti-histone reactivity to be in patients with rheumatoid arthritis with vasculitis, while the highest reactivity in SLE sera was in those patients with a history of photosensitivity. In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. The histones are a group of water and dilute acid soluble basic proteins found associated with DNA in chromosomes. They are characterized by relatively high levels of lysine and arginine. Although histones are classified into a limited number of types of fractions with each particular fraction having a fundamentally distinct amino acid composition and sequence, numerous subfractions are observed due to the acetylation, methylation, and phosphorylation of various amino acid residues. Microheterogeneity or alteration of structure is dynamic such that the histones of a single cell type are found to vary during development. They are believed to play a role in gene activity and cellular metabolism.
Product Categories/Family for anti-H2B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13.8kD
NCBI Official Full Name
histone H2B
NCBI Official Symbol
H2B
NCBI Official Synonym Symbols
MGC159573
NCBI Protein Information
histone H2B
Protein Family
Similar Products
Product Notes
The H2B (Catalog #AAA619758) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Histone H2B reacts with Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF). Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:1000. Researchers should empirically determine the suitability of the H2B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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