Rabbit BLNK Polyclonal Antibody | anti-BLNK antibody

BLNK (Phospho-Y84) Polyclonal Antibody

Cat. Num. AAA3007939

• Gene Names: BLNK; bca; AGM4; BASH; LY57; SLP65; BLNK-S; SLP-65
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: BLNK; Polyclonal Antibody; BLNK (Phospho-Y84) Polyclonal Antibody; B-cell linker protein; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; Cytoplasmic adapter protein; Src homology 2 domain-containing leukocyte protein of 65 kDa; SLP-65; BASH; SLP65; anti-BLNK antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: BLNK (phospho-Y84) polyclonal antibody detects endogenous levels of BLNK protein only when phosphorylated at Try84.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.
• Sequence Length: 433

• Applicable Applications for anti-BLNK antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic phosphopeptide derived from human BLNK around the phosphorylation site of Tyrosine805.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of BLNK (phospho-Y84) polyclonal antibody at 1:500 dilutionLane1:PC12 whole cell lysate (40ug) Lane2:CT26 whole cell lysate (40ug) Lane3:HCT116 whole cell lysate (40ug) Lane4:Jurkat whole cell lysate (40ug) Lane5:Myla2059 whole cell lysate (40ug))

Related Product Information for anti-BLNK antibody

Cross-linking of the B cell receptor (BCR) activates a variety of signaling pathways involved in processes such as cell proliferation and apoptosis. Intracellular protein tyrosine kinases such as Syk and Lyn have been implicated in this BCR signal transduction and are thought to play an important role in B cell development. BLNK (for B cell linker protein) is a central linker protein in B cells which has been shown to associate with the effector proteins GRB2, Vav, Nck and PLCgamma following activation of the B cell receptor. The two forms of BLNK, p68 and p70, arise from alternate splicing of the human BLNK gene transcript. BLNK is phosphorylated by the Syk tyrosine kinase, which in turn permits activation of downstream effector proteins including GRB2 and PLCgamma.

NCBI and Uniprot Product Information

• NCBI GI #: 166235901
• NCBI GeneID: 29760
• NCBI Accession #: NP_001107566.1
• NCBI GenBank Nucleotide #: NP_001107566.1
• UniProt Accession #: Q8WV28
• Molecular Weight: ~ 70kDa
• NCBI Official Full Name: B-cell linker protein isoform 2
• NCBI Official Synonym Full Names: B cell linker
• NCBI Official Symbol: BLNK
• NCBI Official Synonym Symbols: bca; AGM4; BASH; LY57; SLP65; BLNK-S; SLP-65
• NCBI Protein Information: B-cell linker protein
• UniProt Protein Name: B-cell linker protein
• Protein Family: B-cell linker protein
• UniProt Gene Name: BLNK
• UniProt Synonym Gene Names: BASH; SLP65; SLP-65
• UniProt Entry Name: BLNK_HUMAN

NCBI Description

This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

BLNK: an adaptor protein that bridges the B-cell receptor-associated kinases (BCR) with a multitude of signaling pathways, regulating biologic outcomes of B-cell function and development. Plays an important role in BCR-mediated PLCG1 activation and Ca(2 ) mobilization. Does not seem to be not required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Its phosphorylation is required for both Ca(2 ) and MAPK signaling pathways. Defects in BLNK are the cause of hypoglobulinemia and absent B-cells.It has tumor supressor activity that is lost in many cases of childhood pre-B acute lymphoblastic leukemia (ALL). Two alternatively spliced isoforms have been described; these are differentially involved in activation and apoptosis of B lymphocytes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 10q23.2-q23.33

Cellular Component: plasma membrane; intracellular; cytosol

Molecular Function: protein binding; SH3/SH2 adaptor activity; transmembrane receptor protein tyrosine kinase adaptor protein activity

Biological Process: positive regulation of signal transduction; B cell differentiation; inflammatory response; transmembrane receptor protein tyrosine kinase signaling pathway; humoral immune response

Disease: Agammaglobulinemia 4, Autosomal Recessive