Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat lung using H2B K15ac antibody.)
Rabbit Histone H2B Polyclonal Antibody | anti-H2B antibody
Histone H2B (Acetyl-K15) Polyclonal Antibody
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
Histone H2B; Polyclonal Antibody; Histone H2B (Acetyl-K15) Polyclonal Antibody; anti-H2B antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Clonality
Polyclonal
Isotype
IgG
Specificity
Histone H2B (Acetyl-K15) polyclonal antibody detects endogenous levels of Histone H2B protein only when acetylated at Lys15.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-H2B antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IP: 1:50-1:200
IHC: 1:50-1:200
IP: 1:50-1:200
Immunogen
A synthetic acetylated peptide corresponding to residues surrounding K15 of human Histone H2B
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat lung using H2B K15ac antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat lung using H2B K15ac antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human mammary cancer using H2B K15ac antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human mammary cancer using H2B K15ac antibody.)
Related Product Information for anti-H2B antibody
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.
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Product Notes
The H2B (Catalog #AAA3011062) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2B (Acetyl-K15) Polyclonal Antibody reacts with Human, Mouse, Rat, Other (Wide Range) and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC: 1:50-1:200 IP: 1:50-1:200. Researchers should empirically determine the suitability of the H2B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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