Goat BLNK/SLP-65 Polyclonal Antibody | anti-BLNK antibody

Goat anti-BLNK/SLP-65 Antibody

Cat. Num. AAA420310

• Gene Names: BLNK; bca; AGM4; BASH; LY57; SLP65; BLNK-S; SLP-65
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: BLNK/SLP-65; Polyclonal Antibody; Goat anti-BLNK/SLP-65 Antibody; BLNK; SLP-65; B-cell linker; BASH; BLNK-s; Ly57; MGC111051; SLP65; B cell linker protein; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; OTTHUMP00000020167; Src homology 2 domain-containing leuko; BLNK antibody; SLP-65 antibody; B-cell linker antibody; BASH antibody; BLNK-s antibody; Ly57 antibody; MGC111051 antibody; SLP65 antibody; B cell linker protein antibody; B-cell adapter containing a SH2 domain protein antibody; B-cell adapter containing a Src homology 2 domain protein antibody; OTTHUMP00000020167 antibody; Src homology 2 domain-containing leukocyte protein of 65 kDa antibody; BLNK-S antibody; LY57 antibody; OTTHUMP00000020168 antibody; BLNK / SLP-65; anti-BLNK antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: KDSTRLKYAVKVS
• Sequence Length: 456

• Applicable Applications for anti-BLNK antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Western Blot: Approx 70kDa band observed in lysates of cell line Daudi (calculated MW of 50.5kDa according to NP_037446.1). The observed molecular weight corresponds to how SLP-65 is expected to run. However, when transiently expressed in HEK293, a 50kDa band is observed. Recommended concentration: 1-3ug/ml.
• Immunogen: Peptide with sequence C-KDSTRLKYAVKVS, from the C Terminus of the protein sequence according to NP_037446.1; NP_001107566.1.
• Epitope: C Terminus
• Note: This antibody is expected to recognize both reported isoforms (NP_037446.1; NP_001107566.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

(Staining (4ug/ml) of Daudi lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

Western Blot (WB)

(HEK293 overexpressing BLNK (RC202488) and probed (mock transfection in first lane), tested by Origene.)

NCBI and Uniprot Product Information

• NCBI GI #: 7019535
• NCBI GeneID: 29760
• NCBI Accession #: NP_037446.1; NP_001107566.1
• NCBI GenBank Nucleotide #: NM_013314.3
• Molecular Weight: 44,451 Da
• NCBI Official Full Name: B-cell linker protein isoform 1
• NCBI Official Synonym Full Names: B-cell linker
• NCBI Official Symbol: BLNK
• NCBI Official Synonym Symbols: bca; AGM4; BASH; LY57; SLP65; BLNK-S; SLP-65
• NCBI Protein Information: B-cell linker protein
• UniProt Protein Name: B-cell linker protein
• Protein Family: B-cell linker protein
• UniProt Gene Name: BLNK
• UniProt Synonym Gene Names: BASH; SLP65; SLP-65
• UniProt Entry Name: BLNK_HUMAN

NCBI Description

This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

BLNK: an adaptor protein that bridges the B-cell receptor-associated kinases (BCR) with a multitude of signaling pathways, regulating biologic outcomes of B-cell function and development. Plays an important role in BCR-mediated PLCG1 activation and Ca(2 ) mobilization. Does not seem to be not required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Its phosphorylation is required for both Ca(2 ) and MAPK signaling pathways. Defects in BLNK are the cause of hypoglobulinemia and absent B-cells.It has tumor supressor activity that is lost in many cases of childhood pre-B acute lymphoblastic leukemia (ALL). Two alternatively spliced isoforms have been described; these are differentially involved in activation and apoptosis of B lymphocytes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 10q23.2-q23.33

Cellular Component: cytoplasm; cytosol; intracellular; plasma membrane

Molecular Function: protein binding; SH3/SH2 adaptor activity; transmembrane receptor protein tyrosine kinase adaptor protein activity

Biological Process: B cell differentiation; humoral immune response; inflammatory response; positive regulation of signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Agammaglobulinemia 4, Autosomal Recessive