Western Blot (WB)
(Western blot of Histone H2A.J pAb. 20 ug Nuclear Extract of HeLa cells (Lane 1) or 40 ug mouse 3T3-L1 cells before (Lane 2) or following stimulation for 12 days with a defined hormonal mixture (DMSO, dexamethasone, insulin, and IBMX) resulting in differentiation into adipocytes (Lane 3). MW:14kD)
Rabbit anti-Human, Mouse Histone H2A.J Polyclonal Antibody | anti-H2A.J antibody
Histone H2A.J antibody (pAb)
WB: 1:500-1:2500 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot.
Western Blot (WB)
(Western blot of Histone H2A.J pAb. 20 ug Nuclear Extract of HeLa cells (Lane 1) or 40 ug mouse 3T3-L1 cells before (Lane 2) or following stimulation for 12 days with a defined hormonal mixture (DMSO, dexamethasone, insulin, and IBMX) resulting in differentiation into adipocytes (Lane 3). MW:14kD)
Western Blot (WB)
(Western blot of Histone H2A.J pAb. 20 ug Nuclear Extract of HeLa cells (Lane 1) or 40 ug mouse 3T3-L1 cells before (Lane 2) or following stimulation for 12 days with a defined hormonal mixture (DMSO, dexamethasone, insulin, and IBMX) resulting in differentiation into adipocytes (Lane 3). MW:14kD)
Dot Blot (DB)
(Dot blot of Histone H2A.J pAb. Dot blot analysis was used to confirm the specificity of Histone H2A.J antibody. Lane 1: H2A.J peptide. Lane 2: H2A peptide comprised of a.a. 114-126 of human Histone H2A.)
Dot Blot (DB)
(Dot blot of Histone H2A.J pAb. Dot blot analysis was used to confirm the specificity of Histone H2A.J antibody. Lane 1: H2A.J peptide. Lane 2: H2A peptide comprised of a.a. 114-126 of human Histone H2A.)
Background: Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, and is responsible for establishing higher-order chromatin structure. Histone H2A.J is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. Histone H2A.J, which is found only in mammals, is shown to be associated with senescence, accumulating in senescent fibroblasts and promoting expression of inflammatory genes. Therefore, H2A.J may contribute to chronic inflammation and age-related diseases.
NCBI and Uniprot Product Information
NCBI Description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is located on chromosome 12 and encodes a replication-independent histone that is a variant H2A histone. The protein is divergent at the C-terminus compared to the consensus H2A histone family member. This gene also encodes an antimicrobial peptide with antibacterial and antifungal activity.[provided by RefSeq, Oct 2015]
Uniprot Description
H2AFJ: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H2A family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 12p12.3
Cellular Component: nucleosome; nucleus
Molecular Function: DNA binding; protein heterodimerization activity
Research Articles on H2A.J
Similar Products
Product Notes
The H2A.J h2afj (Catalog #AAA389048) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2A.J antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2A.J can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Western Blot (WB). Applications Validated: WB: 1:500-1:2500 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot. Researchers should empirically determine the suitability of the H2A.J h2afj for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2A.J, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
