Immunohistochemistry (IHC)
(Human Small Intestine, MALT: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit Histone H1 Polyclonal Antibody | anti-LOC692942 antibody
Anti-Histone H1 Antibody (N-Terminus)
Reactivity
HumanPredicted: Rabbit, Mouse, Bovine, Rat, Monkey
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Immunoaffinity Purified
Synonyms
Histone H1; Polyclonal Antibody; Anti-Histone H1 Antibody (N-Terminus); Sheep Polyclonal (IgG) to Bovine Histone H1; anti-LOC692942 antibody
Host
Rabbit
Reactivity
Human
Predicted: Rabbit, Mouse, Bovine, Rat, Monkey
Predicted: Rabbit, Mouse, Bovine, Rat, Monkey
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of Histone H1 (pT17) protein.
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, pH 7.3, 0.01% sodium azide, 30% glycerol.
Sequence Length
240
Applicable Applications for anti-LOC692942 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
ICC: 1:100-1:500
IF: 1:100-1:500
IHC-P: 1:100
WB: 1:500-1:1000
IF: 1:100-1:500
IHC-P: 1:100
WB: 1:500-1:1000
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human Histone H1.
Conjugation
Unconjugated
Epitope
N-Terminus
Preparation and Storage
Aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Immunohistochemistry (IHC)
(Human Small Intestine, MALT: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Small Intestine, MALT: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunofluorescence (IF)
(Immunofluorescent analysis of Histone H1 (pT17) staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of Histone H1 (pT17) staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Western Blot (WB)
(Western blot analysis of Histone H1 (pT17) expression in SKOV3 (A); Jurkat (B); COS7 (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Histone H1 (pT17) expression in SKOV3 (A); Jurkat (B); COS7 (C) whole cell lysates.)
Related Product Information for anti-LOC692942 antibody
five main histone protein families which are components of chromatin ineukaryotic cells. Though highly conserved, it is nevertheless the most variable histone in sequence across species. Metazoan H1 protein sfeature a central globular domain and long C- and short N-terminal tails. H1 is involved with the packing of the "beads on a string"sub-structures into a high order structure, whose details have not yetbeen solved.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
histone H1
NCBI Official Symbol
LOC692942
NCBI Protein Information
histone H1
Protein Family
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Product Notes
The LOC692942 (Catalog #AAA2401781) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Histone H1 Antibody (N-Terminus) reacts with Human Predicted: Rabbit, Mouse, Bovine, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H1 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). ICC: 1:100-1:500 IF: 1:100-1:500 IHC-P: 1:100 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the LOC692942 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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