Rabbit Histone Deacetylase 7 Polyclonal Antibody | anti-HDAC7 antibody

Histone Deacetylase 7 (Phospho-S155) polyclonal antibody

Cat. Num. AAA3015095

• Gene Names: HDAC7; HD7A; HDAC7A
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: Histone Deacetylase 7; Polyclonal Antibody; Histone Deacetylase 7 (Phospho-S155) polyclonal antibody; Histone deacetylase 7; HDAC 7; HDAC-7; anti-HDAC7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Histone Deacetylase 7 (Phospho-S155) polyclonal antibody detects endogenous levels of Histone Deacetylase 7 protein only when phosphorylated at Ser155.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-HDAC7 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:200
• Immunogen: Synthetic phosphopeptide derived from human Histone Deacetylase 7 around the phosphorylation site of Serine 155
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of Histone Deacetylase 7 (Phospho-S155)  polyclonal antibody at 1:500 dilutionLaneA: HEK293T whole cell lysateLaneB: Hela whole cell lysateLaneC: U20S whole cell lysateLaneD: The Lung tissue lysate of Rat)

Related Product Information for anti-HDAC7 antibody

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC7 is a histone deacetylase that interacts with the adaptor mSin3A. The interaction of HDAC7 with mSin3A suggests the association of multiple repression complexes of transcription factors.

NCBI and Uniprot Product Information

• NCBI GI #: 148539870
• NCBI GeneID: 51564
• NCBI Accession #: NP_001091886.1
• NCBI GenBank Nucleotide #: NM_001098416.2
• UniProt Accession #: Q8WUI4; Q6P1W9; Q6W9G7; Q7Z4K2; Q7Z5I1; Q96K01; Q9BR73; Q9H7L0; Q9NW41; B3KY08; B4DWI0; B4E0Q5
• Molecular Weight: 102,927 Da
• NCBI Official Full Name: histone deacetylase 7 isoform d
• NCBI Official Synonym Full Names: histone deacetylase 7
• NCBI Official Symbol: HDAC7
• NCBI Official Synonym Symbols: HD7A; HDAC7A
• NCBI Protein Information: histone deacetylase 7; HD7; histone deacetylase 7A
• UniProt Protein Name: Histone deacetylase 7
• Protein Family: Histone deacetylase
• UniProt Gene Name: HDAC7
• UniProt Synonym Gene Names: HDAC7A; HD7; HD7a
• UniProt Entry Name: HDAC7_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC7: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene. Interacts with HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, NCOR1, NCOR2, SIN3A, SIN3B, RBBP4, RBBP7, MTA1L1, SAP30 and MBD3. Interacts with the 14-3-3 protein YWHAE, MEF2A, MEF2B and MEF2C. Interacts with KAT5 and EDNRA. Interacts with KDM5B. Interacts with ZMYND15. Belongs to the histone deacetylase family. HD type 2 subfamily. 10 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.1.98; Hydrolase

Chromosomal Location of Human Ortholog: 12q13.1

Cellular Component: nucleoplasm; cytoplasm; histone deacetylase complex; nucleus

Molecular Function: NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; protein kinase C binding; transcription activator binding; NAD-dependent histone deacetylase activity (H3-K14 specific); metal ion binding; chromatin binding; NAD-dependent histone deacetylase activity (H4-K16 specific); protein kinase binding; transcription corepressor activity

Biological Process: intercellular junction assembly; Notch signaling pathway; transcription, DNA-dependent; negative regulation of osteoblast differentiation; negative regulation of transcription from RNA polymerase II promoter; vasculogenesis; negative regulation of interleukin-2 production