Goat Histamine Receptor H1 Polyclonal Antibody | anti-HRH1 antibody

Goat anti-Histamine Receptor H1 (C Term) Antibody

Cat. Num. AAA420885

• Gene Names: HRH1; H1R; H1-R; HH1R; hisH1
• Reactivity: Human, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Applications: ELISA, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: Histamine Receptor H1; Polyclonal Antibody; Goat anti-Histamine Receptor H1 (C Term) Antibody; Goat Anti-Histamine receptor H1(internal) Antibody; HRH1; histamine receptor H1; HGNC: 5182; H1-R; hisH1; histamine receptor; subclass H1; OTTHUMP00000160133; histamine H(1) receptor; HRH1 antibody; histamine receptor H1 antibody; HGNC: 5182 antibody; H1-R antibody; hisH1 antibody; subclass H1 antibody; OTTHUMP00000160133 antibody; histamine H(1) receptor antibody; Histamine Receptor H1 (C Term); anti-HRH1 antibody

• Host: Goat
• Reactivity: Human, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: CNENFKKTFKRILH
• Sequence Length: 487

• Applicable Applications for anti-HRH1 antibody: Peptide ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Immunohistochemistry: In paraffin embedded Human Colon shows membranous staining. Recommended concentration, 4-6ug/ml.Paraffin embedded Human Small Intestine. Recommended concentration: 5µg/ml. This product has been successfully used in IHC on Rat; Immunofluorescence: This product has been successfully used in IF on Rat; Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
• Immunogen: Peptide with sequence CNENFKKTFKRILH, from the C Terminus of the protein sequence according to NP_000852.1; NP_001091681.1; NP_001091682.1; NP_001091683.1.
• Note: Variants (NP_000852.1; NP_001091681.1; NP_001091682.1; NP_001091683.1) encode the same protein.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((4ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)

Immunohistochemitry (IHC)

((5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining)

NCBI and Uniprot Product Information

• NCBI GI #: 4504491
• NCBI GeneID: 3269
• NCBI Accession #: NP_000852.1; NP_001091681.1; NP_001091682.1; NP_001091683.1
• NCBI GenBank Nucleotide #: NM_000861.3
• NCBI Official Full Name: histamine H1 receptor
• NCBI Official Synonym Full Names: histamine receptor H1
• NCBI Official Symbol: HRH1
• NCBI Official Synonym Symbols: H1R; H1-R; HH1R; hisH1
• NCBI Protein Information: histamine H1 receptor
• UniProt Protein Name: Histamine H1 receptor
• Protein Family: Histamine H1 receptor
• UniProt Gene Name: HRH1
• UniProt Synonym Gene Names: H1R; HH1R
• UniProt Entry Name: HRH1_HUMAN

NCBI Description

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. It has been associated with multiple processes, including memory and learning, circadian rhythm, and thermoregulation. It is also known to contribute to the pathophysiology of allergic diseases such as atopic dermatitis, asthma, anaphylaxis and allergic rhinitis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2015]

Uniprot Description

H1R: In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p25

Cellular Component: cytoplasm; integral to plasma membrane; nucleoplasm; plasma membrane

Molecular Function: G-protein coupled receptor activity; histamine receptor activity

Biological Process: eosinophil chemotaxis; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); inflammatory response; inositol phosphate-mediated signaling; memory; positive regulation of inositol trisphosphate biosynthetic process; positive regulation of vasoconstriction; regulation of synaptic plasticity; regulation of synaptic transmission; regulation of vascular permeability; regulation of vasoconstriction; visual learning