Rabbit anti-Rat Histamine Receptor 3 Polyclonal Antibody | anti-HRH4 antibody

Histamine Receptor 3, CT (HR3)

Cat. Num. AAA6003738

• Gene Names: HRH4; H4; H4R; BG26; HH4R; AXOR35; GPRv53; GPCR105
• Reactivity: Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Histamine Receptor 3; Polyclonal Antibody; CT (HR3); Anti -Histamine Receptor 3; anti-HRH4 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat Histamine Receptor 3 (HR3). The immunogen shows no significant sequence homology with other histamine receptors. Species Crossreactivity
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.

• Applicable Applications for anti-HRH4 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1-10ug/ml using ECL.; ELISA: 1:10,000-1:100,000 using 50-100ng control peptide per well.
• Immunogen: An18aa peptide sequence within the cytoplasmic, C-terminus of rat HR3 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-HRH4 antibody

Histamine, one of the most important mediators of allergy and inflammation, is a chemical messenger and aminergic neurotransmitters. It plays an important role in a multitude of physiological processes in central and peripheral tissues. Histamine is synthesized in a restricted population of neurons located in the tuberomammillary nucleus of the posterior hypothalamus implicated in many brain functions (e.g. sleep/wakefulness, hormonal secretion, cardiovascular control, thermoregulation, food intake, and memory formation). In peripheral tissues histamine is stored in mast cells, basophils, enterochromaffin cells. Histamine release leads to various well-known symptoms of allergic conditions in the skin and the airway system. Histamine effects are mediated by four pharmacologically distinct receptors, the H1R, H2R, H3R and H4R receptors, which are all members of the G-protein coupled receptor (GPCR) family. Histamine receptors display 7 TM domains, an extracellular N-terminus, and a cytoplasmic C-terminus of variable length. Human Histamine 3 receptor, H3R (GPCR97), has recently been identified as a presynaptic autoreceptor on histamine neurons in the brain controlling the stimulated release of histamine. Subsequently, the H3 receptor has been shown to be a presynaptic heteroreceptor in nonhistamine- containing neurons in both the central and peripheral nervous systems. H3R (rat/human 445 aa, ~93% homology) is primarily expressed in the brain. Several studies using H3 selective agonists revealed that H3R couples to pertussis toxin-sensitive Gi/o protein.

Product Categories/Family for anti-HRH4 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 237512996
• NCBI GeneID: 59340
• NCBI Accession #: NP_001153638.1
• NCBI GenBank Nucleotide #: NM_001160166.1
• UniProt Accession #: Q9H3N8; Q4G0I6; Q9GZQ0; B0YJ19; B2KJ48
• Molecular Weight: 44,496 Da
• NCBI Official Full Name: histamine H4 receptor isoform 3
• NCBI Official Synonym Full Names: histamine receptor H4
• NCBI Official Symbol: HRH4
• NCBI Official Synonym Symbols: H4; H4R; BG26; HH4R; AXOR35; GPRv53; GPCR105
• NCBI Protein Information: histamine H4 receptor; SP9144; pfi-013; G-protein coupled receptor 105
• UniProt Protein Name: Histamine H4 receptor
• Protein Family: Histamine H4 receptor
• UniProt Gene Name: HRH4
• UniProt Synonym Gene Names: GPCR105; H4R; HH4R
• UniProt Entry Name: HRH4_HUMAN

NCBI Description

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by a family of histamine receptors, which are a subset of the G-protein coupled receptor superfamily. This gene encodes a histamine receptor that is predominantly expressed in haematopoietic cells. The protein is thought to play a role in inflammation and allergy reponses. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

H4R: The H4 subclass of histamine receptors could mediate the histamine signals in peripheral tissues. Displays a significant level of constitutive activity (spontaneous activity in the absence of agonist). Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: integral to membrane; plasma membrane

Molecular Function: histamine receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; regulation of MAPKKK cascade; inflammatory response