Rabbit anti-Human HIST1H2BJ Polyclonal Antibody | anti-HIST1H2BJ antibody

HIST1H2BJ, ID (HIST1H2BJ, H2BFR, Histone H2B type 1-J, Histone H2B.1, Histone H2B.r) (APC)

Cat. Num. AAA6306825

• Gene Names: HIST1H2BJ; H2BJ; H2B/r; H2BFR
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HIST1H2BJ; Polyclonal Antibody; ID (HIST1H2BJ; H2BFR; Histone H2B type 1-J; Histone H2B.1; Histone H2B.r) (APC); Histone H2B.r; anti-HIST1H2BJ antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-HIST1H2BJ antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: HIST1H2BJ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-90 amino acids from the Central region of human HIST1H2BJ.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(HIST1H2BJ antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIST1H2BJ antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(HIST1H2BJ Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(HIST1H2BJ Antibody (Center) western blot analysis in CEM,HL-60,NCI-H460 cell line lysates (35ug/lane).This demonstrates the HIST1H2BJ antibody detected the HIST1H2BJ protein (arrow).)

Related Product Information for anti-HIST1H2BJ antibody

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq].

Product Categories/Family for anti-HIST1H2BJ antibody

Antibodies; Histones

References

Shi, J., et al. Nature 460(7256):753-757(2009). Benyamin, B., et al. Am. J. Hum. Genet. 84(1):60-65(2009). Kim, S.C., et al. Mol. Cell 23(4):607-618(2006). Beck, H.C., et al. Mol. Cell Proteomics 5(7):1314-1325(2006). Pavri, R., et al. Cell 125(4):703-717(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 20336754
• NCBI GeneID: 8970
• NCBI Accession #: NP_066402.2
• NCBI GenBank Nucleotide #: NM_021058.3
• UniProt Accession #: P06899; O60816; B2R4J4
• Molecular Weight: 13,904 Da
• NCBI Official Full Name: histone H2B type 1-J
• NCBI Official Synonym Full Names: histone cluster 1, H2bj
• NCBI Official Symbol: HIST1H2BJ
• NCBI Official Synonym Symbols: H2BJ; H2B/r; H2BFR
• NCBI Protein Information: histone H2B type 1-J; histone H2B.1; histone H2B.r; histone 1, H2bj; H2B histone family, member R
• UniProt Protein Name: Histone H2B type 1-J
• Protein Family: Histone
• UniProt Gene Name: HIST1H2BJ
• UniProt Synonym Gene Names: H2BFR; H2B/r
• UniProt Entry Name: H2B1J_HUMAN

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Jul 2008]

Uniprot Description

HIST1H2BJ: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Belongs to the histone H2B family.

Chromosomal Location of Human Ortholog: 6p22.1

Cellular Component: nucleoplasm; extracellular space; nucleosome; nucleus

Molecular Function: DNA binding; protein heterodimerization activity

Biological Process: nucleosome assembly; defense response to Gram-positive bacterium; establishment and/or maintenance of chromatin architecture; innate immune response in mucosa; antibacterial humoral response