Western Blot (WB)
(HIRA Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HIRA antibody detected the HIRA protein (arrow).)
Rabbit HIRA Polyclonal Antibody | anti-HIRA antibody
HIRA Antibody (N-term)
Western Blot (WB)
(HIRA Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HIRA antibody detected the HIRA protein (arrow).)
Western Blot (WB)
(HIRA Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HIRA antibody detected the HIRA protein (arrow).)
places the variant histone H3.3 in nucleosomes. Orthologs of this
gene in yeast, flies, and plants are necessary for the formation of
transcriptionally silent heterochomatin. This gene plays an
important role in the formation of the senescence-associated
heterochromatin foci. These foci likely mediate the irreversible
cell cycle changes that occur in senescent cells. It is considered
the primary candidate gene in some haploinsufficiency syndromes
such as DiGeorge syndrome, and insufficient production of the gene
may disrupt normal embryonic development.
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Banumathy, G., et al. Mol. Cell. Biol. 29(3):758-770(2009)
Ramelli, G.P., et al. Dev Med Child Neurol 50(12):953-955(2008)
Zhang, R., et al. Mol. Cell. Biol. 27(6):2343-2358(2007)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development. [provided by RefSeq, Jul 2008]
Uniprot Description
HIRA: a histone chaperone. Necessary to mediate DNA-synthesis-independent nucleosome assembly. Interacts with UBN1, CABIN, and ASF1A in the cell nucleus to form the evolutionarily conserved HUCA histone chaperone complex that deposits the variant histone H3.3 into chromatin in a DNA-replication independent manner. Required for deposition of histone H3.3 at the transcription start sites of genes, where incorporation of histone H3.3 facilitates nucleosome destabilization and contributes to transcriptional activation. Histone H3.3 is also linked to gene silencing and is incorporated into regions of the genome thought to be transcriptionally inactive. While some incorporation of H3.3 into heterochromatin is facilitated by an additional histone chaperone complex containing ATRX and DAXX (ie. telomeric incorporation of H3.3), HIRA is required for incorporation of histone H3.3 and formation of senescence-associated heterochromatin foci (SAHF) during cellular senescence. HIRA is ubiquitously expressed during mouse embryonic development. In the adult mouse, HIRA is expressed at high levels in the kidney, skeletal muscle, and pancreas, but it is expressed at lower levels in the heart, lung, placenta, brain, and liver. A missing copy of the HIRA gene on human chromosome region 22q11.2 is a common characteristic of DiGeorge syndrome patients and insufficient production of the HIRA protein may disrupt normal embryonic development. Two isoforms of the human protein result from alternative splicing.
Protein type: Cell development/differentiation; Transcription regulation; Cell cycle regulation
Chromosomal Location of Human Ortholog: 22q11.21
Cellular Component: nucleoplasm; PML body; protein complex; nuclear chromatin; nucleus
Molecular Function: protein binding; chromatin binding; transcription corepressor activity; transcription factor activity
Biological Process: osteoblast differentiation; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; anatomical structure morphogenesis; DNA replication-independent nucleosome assembly; transcription, DNA-dependent; gastrulation; chromatin modification
Research Articles on HIRA
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Product Notes
The HIRA hira (Catalog #AAA9211903) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 32-61. The HIRA Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's HIRA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the HIRA hira for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIRA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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