Rabbit anti-Human HIRA Polyclonal Antibody | anti-HIRA antibody

HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (S. cerevisiae), HIR, DGCR1, Protein HIRA, TUP1-like Enhancer of Split Protein 1, TUP1, TUPLE1) (HRP)

Cat. Num. AAA6480441

• Gene Names: HIRA; TUP1; DGCR1; TUPLE1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: HIRA; Polyclonal Antibody; HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (S. cerevisiae); HIR; DGCR1; Protein HIRA; TUP1-like Enhancer of Split Protein 1; TUP1; TUPLE1) (HRP); anti-HIRA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HIRA.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 1017

• Applicable Applications for anti-HIRA antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human HIRA (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(HIRA Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the HIRA antibody detected the HIRA protein (arrow).)

Product Categories/Family for anti-HIRA antibody

Antibodies; Histones

References

1. Zhang, R., Mol. Cell. Biol. 27(6), 2343-2358 (2007). 2. Tang, Y., Nat. Struct. Mol. Biol. 13(10), 921-929 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 21536485
• NCBI GeneID: 7290
• NCBI Accession #: NP_003316
• NCBI GenBank Nucleotide #: NP_003316.3
• UniProt Accession #: P54198
• Molecular Weight: 111.835
• NCBI Official Full Name: protein HIRA
• NCBI Official Synonym Full Names: histone cell cycle regulator
• NCBI Official Symbol: HIRA
• NCBI Official Synonym Symbols: TUP1; DGCR1; TUPLE1
• NCBI Protein Information: protein HIRA
• UniProt Protein Name: Protein HIRA
• Protein Family: HIRA-interacting protein
• UniProt Gene Name: HIRA
• UniProt Synonym Gene Names: DGCR1; HIR; TUPLE1
• UniProt Entry Name: HIRA_HUMAN

NCBI Description

This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development. [provided by RefSeq, Jul 2008]

Uniprot Description

HIRA: a histone chaperone. Necessary to mediate DNA-synthesis-independent nucleosome assembly. Interacts with UBN1, CABIN, and ASF1A in the cell nucleus to form the evolutionarily conserved HUCA histone chaperone complex that deposits the variant histone H3.3 into chromatin in a DNA-replication independent manner. Required for deposition of histone H3.3 at the transcription start sites of genes, where incorporation of histone H3.3 facilitates nucleosome destabilization and contributes to transcriptional activation. Histone H3.3 is also linked to gene silencing and is incorporated into regions of the genome thought to be transcriptionally inactive. While some incorporation of H3.3 into heterochromatin is facilitated by an additional histone chaperone complex containing ATRX and DAXX (ie. telomeric incorporation of H3.3), HIRA is required for incorporation of histone H3.3 and formation of senescence-associated heterochromatin foci (SAHF) during cellular senescence. HIRA is ubiquitously expressed during mouse embryonic development. In the adult mouse, HIRA is expressed at high levels in the kidney, skeletal muscle, and pancreas, but it is expressed at lower levels in the heart, lung, placenta, brain, and liver. A missing copy of the HIRA gene on human chromosome region 22q11.2 is a common characteristic of DiGeorge syndrome patients and insufficient production of the HIRA protein may disrupt normal embryonic development. Two isoforms of the human protein result from alternative splicing.

Protein type: Cell development/differentiation; Transcription regulation; Cell cycle regulation

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: nucleoplasm; PML body; protein complex; nuclear chromatin; nucleus

Molecular Function: protein binding; chromatin binding; transcription corepressor activity; transcription factor activity

Biological Process: osteoblast differentiation; regulation of transcription from RNA polymerase II promoter; anatomical structure morphogenesis; muscle cell differentiation; transcription, DNA-dependent; DNA replication-independent nucleosome assembly; gastrulation; chromatin modification