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WB (Western Blot)
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WB (Western Blot) (Detection of human CD40 by western blot. Samples: Whole cell lysate (50 ug) from Raji, HEK293T, U2OS, GaMG, Daudi, A-549, Ramos, HDLM-2, RKO, Jurkat, and MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD40 recombinant monoclonal antibody (AAA23814 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-RPS3 .)

CD40 recombinant antibody

Rabbit anti-CD40 Recombinant Monoclonal Antibody [BLR056F]

Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
CD40; Monoclonal Recombinant Antibody; Rabbit anti-CD40 Recombinant Monoclonal Antibody [BLR056F]; CD40 molecule; TNF receptor superfamily member 5; tumor necrosis factor receptor superfamily member 5; TNFRSF5; p50; CD40L receptor; CD antigen CD40; Bp50; B-cell surface antigen CD40; B cell-associated molecule; B cell surface antigen CD40; CDW40; Tumor necrosis factor receptor superfamily member 5; CD40 recombinant antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR056F
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
250ug/ml (varies by lot)
Applicable Applications for CD40 recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IP: 5 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells.
Immunogen
Between 227 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human CD40 by western blot. Samples: Whole cell lysate (50 ug) from Raji, HEK293T, U2OS, GaMG, Daudi, A-549, Ramos, HDLM-2, RKO, Jurkat, and MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD40 recombinant monoclonal antibody (AAA23814 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-RPS3 .)

WB (Western Blot) (Detection of human CD40 by western blot. Samples: Whole cell lysate (50 ug) from Raji, HEK293T, U2OS, GaMG, Daudi, A-549, Ramos, HDLM-2, RKO, Jurkat, and MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD40 recombinant monoclonal antibody (AAA23814 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-RPS3 .)

IP (Immunoprecipitation)

(Detection of human CD40 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Raji cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD40 recombinant monoclonal antibody (AAA23814 lot 1) used for IP at 5 ul per reaction. CD40 was also immunoprecipitated by rabbit anti-CD40 recombinant monoclonal antibody (BL-790-1G12). For blotting immunoprecipitated CD40, AAA23814 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IP (Immunoprecipitation) (Detection of human CD40 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Raji cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD40 recombinant monoclonal antibody (AAA23814 lot 1) used for IP at 5 ul per reaction. CD40 was also immunoprecipitated by rabbit anti-CD40 recombinant monoclonal antibody (BL-790-1G12). For blotting immunoprecipitated CD40, AAA23814 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IHC (Immunohistochemistry)

(Detection of human CD40 (red) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-CD40 recombinant monoclonal antibody (AAA23814 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)

IHC (Immunohistochemistry) (Detection of human CD40 (red) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-CD40 recombinant monoclonal antibody (AAA23814 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)

IHC (Immunohistochemistry)

(Detection of human CD40 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-CD40 recombinant monoclonal antibody (AAA23814 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry) (Detection of human CD40 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-CD40 recombinant monoclonal antibody (AAA23814 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of human CD40 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-CD40 recombinant monoclonal antibody (AAA23814 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry) (Detection of human CD40 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-CD40 recombinant monoclonal antibody (AAA23814 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human CD40 by immunocytochemistry. Sample: FFPE section of Raji cells. Antibody: Rabbit anti-CD40 recombinant monoclonal antibody (AAA23814 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry) (Detection of human CD40 by immunocytochemistry. Sample: FFPE section of Raji cells. Antibody: Rabbit anti-CD40 recombinant monoclonal antibody (AAA23814 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry)

(Detection of human CD40 (shaded) in Daudi cells by flow cytometry. Antibody: Rabbit anti-CD40 recombinant monoclonal antibody (AAA23814) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of human CD40 (shaded) in Daudi cells by flow cytometry. Antibody: Rabbit anti-CD40 recombinant monoclonal antibody (AAA23814) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for CD40 recombinant antibody
CD40 is a member of the TNF-receptor superfamily. It is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. [provided by RefSeq, Nov 2014] [taken from NCBI Entrez Gene (Gene ID: 958)].
Product Categories/Family for CD40 recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
958
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,619 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 5 isoform 1
NCBI Official Synonym Full Names
CD40 molecule, TNF receptor superfamily member 5
NCBI Official Symbol
CD40
NCBI Official Synonym Symbols
p50; Bp50; CDW40; TNFRSF5
NCBI Protein Information
tumor necrosis factor receptor superfamily member 5; CD40L receptor; CD40 type II isoform; B cell-associated molecule; B cell surface antigen CD40; B-cell surface antigen CD40; CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor recept
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 5
UniProt Gene Name
CD40
UniProt Synonym Gene Names
TNFRSF5
UniProt Entry Name
TNR5_HUMAN

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Product Notes

The CD40 cd40 (Catalog #AAA23814) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CD40 Recombinant Monoclonal Antibody [BLR056F] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD40 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 5 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the CD40 cd40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD40, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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