<
Western Blot (WB)
(Western Blot analysis to detect hippocalcin in Y79 cell lysate (Lane 1) and mouse brain tissue lysate (Lane 2) usingMBS629538.)
Rabbit anti-Human, Mouse Hippocalcin Polyclonal Antibody | anti-Hpca antibody
Hippocalcin, NT (Neuron Specific Calcium-binding Protein Hippocalcin, Calcium-binding Protein BDR-2, Hpca, BDR2) (MaxLight 405)
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A/G Affinity Chromatography.
Synonyms
Hippocalcin; Polyclonal Antibody; NT (Neuron Specific Calcium-binding Protein Hippocalcin; Calcium-binding Protein BDR-2; Hpca; BDR2) (MaxLight 405); anti-Hpca antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Hippocalcin. Species Crossreactivity: mouse
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
Applicable Applications for anti-Hpca antibody
Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 2-32 in the N-terminal region of human Hippocalin, UniProt Accession #P84074. Species sequence homology: bovine, porcine, rat
Conjugate
MaxLight405
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot analysis to detect hippocalcin in Y79 cell lysate (Lane 1) and mouse brain tissue lysate (Lane 2) usingMBS629538.)
Western Blot (WB)
(Western Blot analysis to detect hippocalcin in Y79 cell lysate (Lane 1) and mouse brain tissue lysate (Lane 2) usingMBS629538.)
Western Blot (WB)
(Western Blot analysis of lysate from human brain tissue lysate (35ug), using MBS629538 (1:1000). A goat anti-rabbit IgG H&L(HRP) at 1:5000 was used as the secondary antibody.)
Western Blot (WB)
(Western Blot analysis of lysate from human brain tissue lysate (35ug), using MBS629538 (1:1000). A goat anti-rabbit IgG H&L(HRP) at 1:5000 was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry in formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS629538, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS629538 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Immunohistochemistry in formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS629538, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS629538 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Immunohistochemistry in formalin-fixed and paraffin-embedded human brain tissue reacted withMBS629538, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS629538 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry in formalin-fixed and paraffin-embedded human brain tissue reacted withMBS629538, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS629538 for immunohistochemistry.)
Product Categories/Family for anti-Hpca antibody
References
1. Takamatsu, K., et al., Biochem. Biophys. Res. Commun. 200(1):606-611 (1994). 2. Hidaka, H., et al., Neurosci. Res. 16(2):73-77 (1993). 3. Kobayashi, M., et al., Biochem. Biophys. Res. Commun. 189(1):511-517 (1992). 4. Masaki, T., et al., Gene 225(1-2), 117-124 (1998). 5. Ivings, L., et al., Biochem. J. 363(Pt 3), 599-608 (2002).
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
22.296
Protein Family
Similar Products
Product Notes
The Hpca (Catalog #AAA6480431) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Hippocalcin, NT (Neuron Specific Calcium-binding Protein Hippocalcin, Calcium-binding Protein BDR-2, Hpca, BDR2) (MaxLight 405) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Hippocalcin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Hpca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hippocalcin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
