Rabbit anti-Human Pax 2 Polyclonal Antibody | anti-Pxn antibody

Pax 2, phosphorylated (Paired Box Gene 2, Paired Box Homeotic Gene 2, Paired Box Protein 2, Paired Box Protein Pax-2, Paired Box Protein Pax2, Pax-2)

Cat. Num. AAA630929

• Gene Names: Pxn; Pax; AW108311; AW123232
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Pax 2; Polyclonal Antibody; phosphorylated (Paired Box Gene 2; Paired Box Homeotic Gene 2; Paired Box Protein 2; Paired Box Protein Pax-2; Paired Box Protein Pax2; Pax-2); Anti -Pax 2; anti-Pxn antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Pax2 protein when phosphorylated at Ser393. Based on amino acid sequence homology with Pax5 and Pax8, crossreactivity with these proteins is likely. Does not react with puri ed, unphosphorylated GST-Pax2 proteins. Reacts with human HEK293 cell lysates and puri ed GST-Pax2 proteins phosphorylated by JNK.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-Pxn antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 0.1-1ug/ml; Western Blot: 1-3ug/ml; Western Blot analysis of JNK-stimulated (phosphorylated) fusion proteins identi es a single band at ~50kD. In Western Blot analysis of HEK293 cells, a thick band between ~42-45kD is observed, which may represent different human Pax2 isoforms and/or Pax8.
• Immunogen: Synthetic phospho-peptide derived from the sequence surrounding the phosphorylated Ser393 residue of mouse and human Pax2 (paired box protein 2). Species Sequence Homology: mouse, chicken, zebra?sh and frog - 100%.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot of analysis of (A) phospho-Pax2-GST fusion protein, (B) Pax2-GST fusion protein, and (C) HEK293 cell lysates using MBS630929.)

Related Product Information for anti-Pxn antibody

Pax2 is a transcription factor encoded by a gene that is required for the embryonic development of a variety of mammalian tissues, including the kidney, optic cup, and inner ear (1,2). Structurally, the Pax2/5/8 protein subfamily is characterized by an N-terminal paired domain, a conserved octapeptide sequence, and a paired-paired type homeobox. The N-terminal paired domain of the Pax2/5/8 proteins binds DNA, while the C-terminal region is required for activation of target genes (3). In human mammary tissue, Pax2 expression has been identified in sub-populations of mammary ductal cells, and also in > 50% of human breast tumors surveyed (n=38) (4). Mouse studies have demonstrated co-localization of Pax2 and WT1 (Wilms' tumor suppressor protein) in the nuclei of normal and cancerous breast cells, indicating a role for Pax2 in the regulation of the response to progesterone in the mature mammary gland (4).

Product Categories/Family for anti-Pxn antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 114326502
• NCBI GeneID: 19303
• NCBI Accession #: NP_598676.2
• NCBI GenBank Nucleotide #: NM_133915.3
• UniProt Accession #: Q8VI36; Q3TB62; Q3TZQ6; Q8VI37
• Molecular Weight: 64,476 Da
• NCBI Official Full Name: paxillin isoform beta
• NCBI Official Synonym Full Names: paxillin
• NCBI Official Symbol: Pxn
• NCBI Official Synonym Symbols: Pax; AW108311; AW123232
• NCBI Protein Information: paxillin
• UniProt Protein Name: Paxillin
• Protein Family: Paxillin
• UniProt Gene Name: Pxn
• UniProt Entry Name: PAXI_MOUSE

Uniprot Description

Function: Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion)

By similarity.

Subunit structure: Interacts with VCL and SRC (via SH3 domain). Interacts with GIT1, NUDT16L1/SDOS and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds ASAP2. Interacts with RNF5 and PDCD10

By similarity. Interacts with SORBS1, PARVA and PARVB. Interacts with NEK3 and this interaction is prolactin-dependent

By similarity. Interacts with PTK2/FAK1. Interacts with PTK6

By similarity. Interacts with PTK2B/PYK2. Ref.3 Ref.5 Ref.6

Subcellular location: Cytoplasm › cytoskeleton

By similarity. Cell junction › focal adhesion

By similarity. Cytoplasm › cell cortex. Note: Colocalizes with integrins at the cell periphery. Ref.3

Post-translational modification: Phosphorylated by MAPK1/ERK2. Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation

By similarity. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion

By similarity. Ref.4 Ref.10

Sequence similarities: Belongs to the paxillin family.Contains 4 LIM zinc-binding domains.