Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. small intestine section using HIP2 Antibody (Center). MBS9213324 was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Rabbit HIP2 Polyclonal Antibody | anti-UBE2K antibody
HIP2 Antibody (Center)
IF~~1:10~50
WB~~1:1000
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. small intestine section using HIP2 Antibody (Center). MBS9213324 was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. small intestine section using HIP2 Antibody (Center). MBS9213324 was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Immunofluorescence (IF)
(Fluorescent image of Hela cell stained with HIP2 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HIP2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). HIP2 immunoreactivity is localized to Cytoplasm significantly.)
Immunofluorescence (IF)
(Fluorescent image of Hela cell stained with HIP2 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HIP2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). HIP2 immunoreactivity is localized to Cytoplasm significantly.)
Western Blot (WB)
(HIP2 Antibody (D124) western blot analysis in 293,mouse C2C12 cell line lysates (35ug/lane).This demonstrates the HIP2 antibody detected the HIP2 protein (arrow).)
Western Blot (WB)
(HIP2 Antibody (D124) western blot analysis in 293,mouse C2C12 cell line lysates (35ug/lane).This demonstrates the HIP2 antibody detected the HIP2 protein (arrow).)
Kikuchi, J., et al., Arterioscler. Thromb. Vasc. Biol. 20(1):128-134 (2000).
Petersen, A., et al., Exp. Neurol. 157(1):1-18 (1999).
Kalchman, M.A., et al., J. Biol. Chem. 271(32):19385-19394 (1996).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
UBE2K: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein- dependent degradation of RB1. Interacts with RNF138/NARF. Interacts with BRCA1. By aggregated low-density lipoprotein. Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem. Belongs to the ubiquitin-conjugating enzyme family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 6.3.2.19; Ligase; Ubiquitin ligase; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 4p14
Cellular Component: intermediate filament cytoskeleton; cytoplasm; nucleus; actin cytoskeleton
Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity
Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; regulation of proteasomal ubiquitin-dependent protein catabolic process
Research Articles on UBE2K
Similar Products
Product Notes
The UBE2K ube2k (Catalog #AAA9213324) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 109-139. The HIP2 Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's HIP2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), Immunofluorescence (IF). IHC~~1:100 IF~~1:10~50 WB~~1:1000. Researchers should empirically determine the suitability of the UBE2K ube2k for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
