Rabbit UBE2K Polyclonal Antibody | anti-UBE2K antibody

UBE2K antibody

Cat. Num. AAA9412709

• Gene Names: UBE2K; LIG; HIP2; HYPG; UBC1; E2-25K
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: UBE2K; Polyclonal Antibody; UBE2K antibody; DKFZp564C1216; DKFZp686J24237; E2-25K; HIP2; HYPG; LIG; anti-UBE2K antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total UBE2K antibody.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 157

• Applicable Applications for anti-UBE2K antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500 - 1:2000
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human UBE2K.
• Target Name: UBE2K
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of human brain cell lysate using UBE2K antibody.)

Related Product Information for anti-UBE2K antibody

Protein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an eta-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3 (1).

Product Categories/Family for anti-UBE2K antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 163660385
• NCBI GeneID: 3093
• NCBI Accession #: NP_001104582.1
• NCBI GenBank Nucleotide #: NM_001111112.1
• UniProt Accession #: P61086; O54806; P27924; Q16721; Q9CVV9; Q9Y2D3; A6NJC1; A8K5Y9; B2RDF8; C9JGP1
• Molecular Weight: 17,447 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 K isoform 2
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2K
• NCBI Official Symbol: UBE2K
• NCBI Official Synonym Symbols: LIG; HIP2; HYPG; UBC1; E2-25K
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 K
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 K
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2K
• UniProt Synonym Gene Names: HIP2; LIG; HIP-2; Ubiquitin-conjugating enzyme E2(25K); Ubiquitin-conjugating enzyme E2-25K
• UniProt Entry Name: UBE2K_HUMAN

NCBI Description

The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

UBE2K: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein- dependent degradation of RB1. Interacts with RNF138/NARF. Interacts with BRCA1. By aggregated low-density lipoprotein. Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem. Belongs to the ubiquitin-conjugating enzyme family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.19; Ubiquitin conjugating system; Ligase; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: intermediate filament cytoskeleton; cytoplasm; nucleus; actin cytoskeleton

Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; regulation of proteasomal ubiquitin-dependent protein catabolic process