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IF (Immunofluorescence) (Immunofluorescent analysis of MCF-7 cells using AAA18542 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Rabbit anti-Human Glyceraldehyde-3-phosphate dehydrogenase Polyclonal Antibody | anti-GAPDH antibody

Rabbit anti-human Glyceraldehyde-3-phosphate dehydrogenase polyclonal Antibody

Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
Glyceraldehyde-3-phosphate dehydrogenase; Polyclonal Antibody; Rabbit anti-human Glyceraldehyde-3-phosphate dehydrogenase polyclonal Antibody; Glyceraldehyde-3-phosphate dehydrogenase protein; GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Peptidyl-cysteine S-nitrosylase GAPDH; anti-GAPDH antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
293
Applicable Applications for anti-GAPDH antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Recommended dilution: WB:1:500-2000, IHC:1:20-1:200
Immunogen
Recombinant human Glyceraldehyde-3-phosphate dehydrogenase protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-20357 / sc-20356 / sc-20358 / sc-48167 / sc-31915 / sc-48166 / sc-25778
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

IF (Immunofluorescence)

(Immunofluorescent analysis of MCF-7 cells using AAA18542 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IF (Immunofluorescence) (Immunofluorescent analysis of MCF-7 cells using AAA18542 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human pancreatic tissue using AAA18542 at dilution of 1:100)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human pancreatic tissue using AAA18542 at dilution of 1:100)

IHC (Immunohistchemistry)

(Immunohistochemistry of paraffin-embedded human prostate cancer using AAA18542 at dilution of 1:100)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human prostate cancer using AAA18542 at dilution of 1:100)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded mouse liver using AAA18542 at dilution 1:50)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using AAA18542 at dilution 1:50)

WB (Western Blot)

(Western BlotPositive WB detected in:Hela whole cell lysate,A549 whole cell lysate,HEK293 whole cell lysate,HepG2 whole cell lysate,Jurkat whole cell lysate,K562 whole cell lysateAll lanes: GAPDH antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)

WB (Western Blot) (Western BlotPositive WB detected in:Hela whole cell lysate,A549 whole cell lysate,HEK293 whole cell lysate,HepG2 whole cell lysate,Jurkat whole cell lysate,K562 whole cell lysateAll lanes: GAPDH antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)

WB (Western Blot)

(Western BlotPositive WB detected in: hepG2 cell,mouse brain,mouse skeletal muscleAll lanes: GAPDH antibody at 2.6ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)

WB (Western Blot) (Western BlotPositive WB detected in: hepG2 cell,mouse brain,mouse skeletal muscleAll lanes: GAPDH antibody at 2.6ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)

WB (Western Blot)

(Western blotAll lanes: GAPDH antibody at 2ug/mlLane 1: Hela whole cell lysateLane 2: 293T whole cell lysateLane 3: Jurkat whole cell lysateLane 4: Mouse kidney tissueLane 5: Rat muscle tissueLane 6: Rat lung tissueLane 7: Zebrafish lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)

WB (Western Blot) (Western blotAll lanes: GAPDH antibody at 2ug/mlLane 1: Hela whole cell lysateLane 2: 293T whole cell lysateLane 3: Jurkat whole cell lysateLane 4: Mouse kidney tissueLane 5: Rat muscle tissueLane 6: Rat lung tissueLane 7: Zebrafish lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)

WB (Western Blot)

(Western blotAll lanes: Glyceraldehyde-3-phosphate dehydrogenase antibody at 2ug/mlLane 1: Hale whole cell lysateLane 2: A549 whole cell lysateLane 3: HepG2 whole cell lysateLane 4: K562 whole cell lysateLane 5: Jurkats whole cell lysateLane 6: MDA-MB-231 whole cell lysateLane 7: LO2 whole cell lysateLane 8: U251 whole cell lysateLane 9: MCF-7 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)

WB (Western Blot) (Western blotAll lanes: Glyceraldehyde-3-phosphate dehydrogenase antibody at 2ug/mlLane 1: Hale whole cell lysateLane 2: A549 whole cell lysateLane 3: HepG2 whole cell lysateLane 4: K562 whole cell lysateLane 5: Jurkats whole cell lysateLane 6: MDA-MB-231 whole cell lysateLane 7: LO2 whole cell lysateLane 8: U251 whole cell lysateLane 9: MCF-7 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)
Related Product Information for anti-GAPDH antibody
Glycerol 3 Phosphate Dehydrogenase is a homodimer and belongs to the NAD dependent Glycerol 3 Phosphate Dehydrogenase family.

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.
Product Categories/Family for anti-GAPDH antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31,548 Da
NCBI Official Full Name
glyceraldehyde-3-phosphate dehydrogenase isoform 2
NCBI Official Synonym Full Names
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol
GAPDH
NCBI Official Synonym Symbols
G3PD; GAPD; HEL-S-162eP
NCBI Protein Information
glyceraldehyde-3-phosphate dehydrogenase
UniProt Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
UniProt Gene Name
GAPDH
UniProt Synonym Gene Names
GAPD; GAPDH
UniProt Entry Name
G3P_HUMAN

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Product Notes

The GAPDH gapdh (Catalog #AAA18542) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Glyceraldehyde-3-phosphate dehydrogenase polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Glyceraldehyde-3-phosphate dehydrogenase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Recommended dilution: WB:1:500-2000, IHC:1:20-1:200. Researchers should empirically determine the suitability of the GAPDH gapdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glyceraldehyde-3-phosphate dehydrogenase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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