Western Blot (WB)
(Western Blot: Sample: Rat Heart lysate; Primary Ab: 3ug/ml Rabbit Anti-Mouse EPYC Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Epiphycan Polyclonal Antibody | anti-EPYC antibody
Polyclonal Antibody to Epiphycan (EPYC)
Gene Names
Epyc; Dspg3; PG-Lb; SLRR3B
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Epiphycan; Polyclonal Antibody; Polyclonal Antibody to Epiphycan (EPYC); anti-EPYC antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against EPYC. It has beenselected for its ability to recognize EPYC in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- C DDHELDAIPP LPKKTTYFYS RFNRIKKINK NDFASLNDLK RIDLTSNLIS EIDEDAFRKL PHLQELVLRD NKIKQLPELP NTLTFIDISN NRLGRKGIKQ EAFKDMYDLH HLYITDNSLD HIPLPLPESL RALHLQNNDI
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- C DDHELDAIPP LPKKTTYFYS RFNRIKKINK NDFASLNDLK RIDLTSNLIS EIDEDAFRKL PHLQELVLRD NKIKQLPELP NTLTFIDISN NRLGRKGIKQ EAFKDMYDLH HLYITDNSLD HIPLPLPESL RALHLQNNDI
Sequence Length
322
Applicable Applications for anti-EPYC antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
EPYC (Cys130~Ile322)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse, Rat
Conjugate
No Conjugate
Immunogen
Recombinant EPYC (Cys130~Ile322) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2080992
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Rat Heart lysate; Primary Ab: 3ug/ml Rabbit Anti-Mouse EPYC Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Rat Heart lysate; Primary Ab: 3ug/ml Rabbit Anti-Mouse EPYC Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded testis tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded testis tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,763 Da
NCBI Official Full Name
epiphycan
NCBI Official Synonym Full Names
epiphycan
NCBI Official Symbol
Epyc
NCBI Official Synonym Symbols
Dspg3; PG-Lb; SLRR3B
NCBI Protein Information
epiphycan
UniProt Protein Name
Epiphycan
Protein Family
UniProt Gene Name
Epyc
UniProt Synonym Gene Names
Dspg3; Pglb; PG-Lb
Uniprot Description
EPYC: May have a role in bone formation and also in establishing the ordered structure of cartilage through matrix organization. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class III subfamily.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 10 C3|10 50.35 cM
Cellular Component: extracellular region; extracellular space; proteinaceous extracellular matrix
Biological Process: axonogenesis
Research Articles on EPYC
Similar Products
Product Notes
The EPYC epyc (Catalog #AAA2027323) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Epiphycan (EPYC) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Epiphycan can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the EPYC epyc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- C DDHELDAIPP LPKKTTYFYS RFNRIKKINK NDFASLNDLK RIDLTSNLIS EIDEDAFRKL PHLQELVLRD NKIKQLPELP NTLTFIDISN NRLGRKGIKQ EAFKDMYDLH HLYITDNSLD HIPLPLPESL RALHLQNNDI. It is sometimes possible for the material contained within the vial of "Epiphycan, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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