Rabbit HINT / HINT1 Polyclonal Antibody | anti-HINT antibody

Rabbit Polyclonal to Human HINT / HINT1

Cat. Num. AAA244644

• Gene Names: HINT1; HINT; NMAN; PKCI-1; PRKCNH1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: HINT / HINT1; Polyclonal Antibody; Rabbit Polyclonal to Human HINT / HINT1; Anti-HINT / HINT1 Antibody (Internal) IHC-plus; HINT1; HINT; NMAN; Protein kinase C inhibitor 1; PKCI-1; PKCI1; PRKCNH1; Human HINT; anti-HINT antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 126

• Applicable Applications for anti-HINT antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (5 ug/ml), WB (1 - 2 ug/ml); Usage: HINT1 antibody can be used for detection of HINT1 by Western blot at 1-2 ug/ml
• Target Species: Human
• Immunogen Description: A 17 amino acid peptide near the center of human HINT1.
• Immunogen Type: Synthetic peptide
• Immunogen: HINT / HINT1 antibody was raised against a 17 amino acid peptide near the center of human HINT1.
• Antigen Modification: Internal
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-HINT1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunocytochemistry (ICC)

(Immunocytochemistry of HINT1 in Jurkat cells with HINT1 antibody at 2.5 ug/ml.)

Western Blot (WB)

(Western blot of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 4885413
• NCBI GeneID: 3094
• NCBI Accession #: NP_005331.1
• NCBI GenBank Nucleotide #: NM_005340.6
• UniProt Accession #: P49773; Q9H5W8
• Molecular Weight: 13,802 Da
• NCBI Official Full Name: histidine triad nucleotide-binding protein 1
• NCBI Official Synonym Full Names: histidine triad nucleotide binding protein 1
• NCBI Official Symbol: HINT1
• NCBI Official Synonym Symbols: HINT; NMAN; PKCI-1; PRKCNH1
• NCBI Protein Information: histidine triad nucleotide-binding protein 1; protein kinase C inhibitor 1; adenosine 5'-monophosphoramidase; protein kinase C-interacting protein 1
• UniProt Protein Name: Histidine triad nucleotide-binding protein 1
• UniProt Gene Name: HINT1
• UniProt Synonym Gene Names: HINT; PKCI1; PRKCNH1; PKCI-1
• UniProt Entry Name: HINT1_HUMAN

NCBI Description

The protein encoded by this gene can hydrolyze substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif, which is part of the loop that binds to the substrate. This gene has been found to be a tumor suppressing gene. Several transcript variants, but only one of them protein-coding, have been found for this gene. [provided by RefSeq, Dec 2012]

Uniprot Description

HINT1: Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha- acetyl lysine methyl ester and AMP-NH2. Belongs to the HINT family.

Protein type: Hydrolase; EC 3.-.-.-; Cytoskeletal

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: cytoskeleton; histone deacetylase complex; cytoplasm; plasma membrane; nucleus

Molecular Function: protein kinase C binding; hydrolase activity; nucleotide binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; purine ribonucleotide catabolic process; signal transduction

Disease: Neuromyotonia And Axonal Neuropathy, Autosomal Recessive