Rabbit anti-Human HIC1 Polyclonal Antibody | anti-HIC1 antibody

HIC1, CT (HIC1, ZBTB29, Hypermethylated in cancer 1 protein, Zinc finger and BTB domain-containing protein 29) (AP)

Cat. Num. AAA6306428

• Gene Names: HIC1; hic-1; ZBTB29; ZNF901
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HIC1; Polyclonal Antibody; CT (HIC1; ZBTB29; Hypermethylated in cancer 1 protein; Zinc finger and BTB domain-containing protein 29) (AP); Zinc finger and BTB domain-containing protein 29; anti-HIC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-HIC1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: HIC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 497-527 amino acids from the C-terminal region of human HIC1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(HIC1 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the HIC1 antibody detected the HIC1 protein (arrow).)

Related Product Information for anti-HIC1 antibody

This gene functions as a growth regulatory and tumor repressor gene. Hypermethylation or deletion of the region of this gene have been associated with tumors and the contiguous-gene syndrome, Miller-Dieker syndrome. Alternative splicing of this gene results in multiple transcript variants.

Product Categories/Family for anti-HIC1 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Pehlivan, S., et al. Cancer Genet. Cytogenet. 201(2):128-132(2010). Van Rechem, C., et al. Mol. Cell. Biol. 30(16):4045-4059(2010). Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010): Zhang, B., et al. Mol. Endocrinol. 23(12):2075-2085(2009). Tseng, R.C., et al. Neoplasia 11(8):763-770(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 148237270
• NCBI GeneID: 3090
• NCBI Accession #: NP_001091672.1; NP_006488.2
• NCBI GenBank Nucleotide #: NM_001098202.1
• UniProt Accession #: Q14526; D3DTI4
• Molecular Weight: 76,508 Da
• NCBI Official Full Name: hypermethylated in cancer 1 protein isoform 2
• NCBI Official Synonym Full Names: hypermethylated in cancer 1
• NCBI Official Symbol: HIC1
• NCBI Official Synonym Symbols: hic-1; ZBTB29; ZNF901
• NCBI Protein Information: hypermethylated in cancer 1 protein; zinc finger and BTB domain-containing protein 29
• UniProt Protein Name: Hypermethylated in cancer 1 protein
• Protein Family: Hypermethylated in cancer 1 protein
• UniProt Gene Name: HIC1
• UniProt Synonym Gene Names: ZBTB29; Hic-1
• UniProt Entry Name: HIC1_HUMAN

NCBI Description

This gene functions as a growth regulatory and tumor repressor gene. Hypermethylation or deletion of the region of this gene have been associated with tumors and the contiguous-gene syndrome, Miller-Dieker syndrome. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2010]

Uniprot Description

HIC1: Transcriptional repressor. Recognizes and binds to the consensus sequence '5-[CG]NG[CG]GGGCA[CA]CC-3'. May act as a tumor suppressor. May be involved in development of head, face, limbs and ventral body wall. Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses. The specific target gene promoter association seems to be depend on corepressors, such as CTBP1 or CTBP2 and MTA1. The regulation of SIRT1 transcription in response to nutrient deprivation seems to involve CTBP1. In cooperation with MTA1 (indicative for an association with the NuRD complex) represses transcription from CCND1/cyclin-D1 and CDKN1C/p57Kip2 specifically in quiescent cells. Involved in regulation of the Wnt signaling pathway probably by association with TCF7L2 and preventing TCF7L2 and CTNNB1 association with promoters of TCF- responsive genes. Seems to repress transcription from E2F1 and ATOH1 which involves ARID1A, indicative for the participation of a distinct SWI/SNF-type chromatin-remodeling complex. Probably represses transcription from CXCR7, FGFBP1 and EFNA1. Belongs to the krueppel C2H2-type zinc-finger protein family. Hic subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Tumor suppressor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: nucleoplasm; cytoplasm; chromatin

Molecular Function: protein binding; sequence-specific DNA binding; metal ion binding; histone deacetylase binding; transcription factor activity

Biological Process: negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; regulation of transcription, DNA-dependent; DNA damage response, signal transduction resulting in induction of apoptosis; transcription, DNA-dependent; multicellular organismal development; positive regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of transcription from RNA polymerase II promoter