Rabbit anti-Human HIBADH Polyclonal Antibody | anti-HIBADH antibody

HIBADH, NT (HIBADH, 3-hydroxyisobutyrate dehydrogenase, mitochondrial) (Biotin)

Cat. Num. AAA6306419

• Gene Names: HIBADH; NS5ATP1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HIBADH; Polyclonal Antibody; NT (HIBADH; 3-hydroxyisobutyrate dehydrogenase; mitochondrial) (Biotin); mitochondrial; anti-HIBADH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-HIBADH antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: HIBADH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIBADH.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HIBADH Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the HIBADH antibody detected the HIBADH protein (arrow).)

Product Categories/Family for anti-HIBADH antibody

Antibodies; Mitochondrial Proteins

References

Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009): Rougraff, P.M., et al. J. Biol. Chem. 264(10):5899-5903(1989) Rougraff, P.M., et al. J. Biol. Chem. 263(1):327-331(1988) ROBINSON, W.G., et al. J. Biol. Chem. 225(1):511-521(1957).

NCBI and Uniprot Product Information

• NCBI GI #: 23308751
• NCBI GeneID: 11112
• NCBI Accession #: NP_689953.1
• NCBI GenBank Nucleotide #: NM_152740.3
• UniProt Accession #: P31937; Q546Z2; Q9UDN3
• Molecular Weight: 35,329 Da
• NCBI Official Full Name: 3-hydroxyisobutyrate dehydrogenase, mitochondrial
• NCBI Official Synonym Full Names: 3-hydroxyisobutyrate dehydrogenase
• NCBI Official Symbol: HIBADH
• NCBI Official Synonym Symbols: NS5ATP1
• NCBI Protein Information: 3-hydroxyisobutyrate dehydrogenase, mitochondrial; 3'-hydroxyisobutyrate dehydrogenase
• UniProt Protein Name: 3-hydroxyisobutyrate dehydrogenase, mitochondrial
• Protein Family: 3-hydroxyisobutyrate dehydrogenase
• UniProt Gene Name: HIBADH
• UniProt Synonym Gene Names: HIBADH
• UniProt Entry Name: 3HIDH_HUMAN

NCBI Description

This gene encodes a mitochondrial 3-hydroxyisobutyrate dehydrogenase enzyme. The encoded protein plays a critical role in the catabolism of L-valine by catalyzing the oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde. [provided by RefSeq, Nov 2011]

Uniprot Description

HIBADH: a mitochondrial 3-hydroxyisobutyrate dehydrogenase enzyme. The encoded protein plays a critical role in the catabolism of L-valine by catalyzing the oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde. [provided by RefSeq, Nov 2011]

Protein type: EC 1.1.1.31; Mitochondrial; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Oxidoreductase

Chromosomal Location of Human Ortholog: 7p15.2

Cellular Component: mitochondrial matrix

Molecular Function: phosphogluconate dehydrogenase (decarboxylating) activity; 3-hydroxyisobutyrate dehydrogenase activity; NAD binding

Biological Process: pentose-phosphate shunt; valine catabolic process; branched chain family amino acid catabolic process