Western Blot (WB)
(Western blot analysis of extracts ofK-562 cells, using HFM1 antibody (MBS9129805) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit HFM1 Polyclonal Antibody | anti-HFM1 antibody
HFM1 Polyclonal Antibody
IHC: 1:50- 1:100
Western Blot (WB)
(Western blot analysis of extracts ofK-562 cells, using HFM1 antibody (MBS9129805) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts ofK-562 cells, using HFM1 antibody (MBS9129805) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Immunohistochemistry
(Immunohistochemistry of paraffin-embedded mouse testis using HFM1antibody (MBS9129805) at dilution of 1:100(40x lens).)
Immunohistochemistry
(Immunohistochemistry of paraffin-embedded mouse testis using HFM1antibody (MBS9129805) at dilution of 1:100(40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat testis using HFM1antibody (MBS9129805) at dilution of 1:100(40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat testis using HFM1antibody (MBS9129805) at dilution of 1:100(40x lens).)
NCBI and Uniprot Product Information
Observed MW: 130kDa
NCBI Description
The protein encoded by this gene is thought to be an ATP-dependent DNA helicase and is expressed mainly in germ-line cells. Defects in this gene are a cause of premature ovarian failure 9 (POF9). [provided by RefSeq, Apr 2014]
Uniprot Description
SEC63D1: Belongs to the helicase family. SKI2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; EC 3.6.4.12; Helicase
Chromosomal Location of Human Ortholog: 1p22.2
Molecular Function: ATP binding; helicase activity; nucleic acid binding
Biological Process: resolution of meiotic joint molecules as recombinants
Disease: Premature Ovarian Failure 9
Research Articles on HFM1
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Product Notes
The HFM1 hfm1 (Catalog #AAA9129805) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HFM1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500 - 1:2000 IHC: 1:50- 1:100. Researchers should empirically determine the suitability of the HFM1 hfm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HFM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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