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Testing Data (Primary: Anti-HFH4 at 1/1000 dilution)

Rabbit HFH4 Polyclonal Antibody | anti-HFH4 antibody

HFH4 Polyclonal Antibody

Gene Names
FOXJ1; HFH4; HFH-4; FKHL13
Reactivity
Human, Mouse, Rat, Dog, Cow, Rabbit
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
affinity purified by Protein A
Synonyms
HFH4; Polyclonal Antibody; HFH4 Polyclonal Antibody; Forkhead box protein J1; FOXJ-1; winged helix; FOXJ1; FKHL13; HFH-4; MGC35202; forkhead box J1; Forkhead Homologue 4; Forkhead related protein FKHL13; Fox J1; Hepatocyte nuclear factor 3 forkhead homolog 4; HNF-3/forkhead homolog 4; FOXJ1_HUMAN; anti-HFH4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Cow, Rabbit
Clonality
Polyclonal
Specificity
HFH4 Polyclonal Antibody detects endogenous levels of HFH4 protein.
Purity/Purification
affinity purified by Protein A
Form/Format
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 56% Glycerol.
Applicable Applications for anti-HFH4 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM)
Application Notes
WB: 1:500-2000
IHC-P: 1:100-500
FC/FACS/FCM: 0.2ug/Test
Immunogen
KLH conjugated synthetic peptide derived from human FOXJ1:161-260/421
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -25 degree C long term. Avoid freeze-thaw cycles.

Testing Data

(Primary: Anti-HFH4 at 1/1000 dilution)

Testing Data (Primary: Anti-HFH4 at 1/1000 dilution)

Testing Data

(Primary: Anti-HFH4 at 1/500 dilution)

Testing Data (Primary: Anti-HFH4 at 1/500 dilution)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HFH4) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C)

Immunohistochemistry (IHC) (Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HFH4) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C)
Related Product Information for anti-HFH4 antibody
This gene encodes a member of the forkhead family of transcription factors. Similar genes in zebrafish and mouse have been shown to regulate the transcription of genes that control the production of motile cilia. The mouse ortholog also functions in the determination of left-right asymmetry. Polymorphisms in this gene are associated with systemic lupus erythematosus and allergic rhinitis.[provided by RefSeq, Sep 2009]

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
45,247 Da
NCBI Official Full Name
forkhead box protein J1
NCBI Official Synonym Full Names
forkhead box J1
NCBI Official Symbol
FOXJ1
NCBI Official Synonym Symbols
HFH4; HFH-4; FKHL13
NCBI Protein Information
forkhead box protein J1; forkhead-like 13; fork head homologue 4; forkhead-related protein FKHL13; forkhead transcription factor HFH-4; hepatocyte nuclear factor 3 forkhead homolog 4
UniProt Protein Name
Forkhead box protein J1
UniProt Gene Name
FOXJ1
UniProt Synonym Gene Names
FKHL13; HFH4; HFH-4
UniProt Entry Name
FOXJ1_HUMAN

NCBI Description

This gene encodes a member of the forkhead family of transcription factors. Similar genes in zebrafish and mouse have been shown to regulate the transcription of genes that control the production of motile cilia. The mouse ortholog also functions in the determination of left-right asymmetry. Polymorphisms in this gene are associated with systemic lupus erythematosus and allergic rhinitis.[provided by RefSeq, Sep 2009]

Uniprot Description

FOXJ1: May play an important role in cell fate determination during lung development and in spermatogenesis. Genetic variations in FOXJ1 may be associated with susceptibility to allergic rhinitis (ALRH). Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: nucleus

Molecular Function: DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; negative regulation of germinal center formation; positive regulation of central B cell tolerance induction; establishment of apical/basal cell polarity; negative regulation of B cell activation; negative regulation of interleukin-6 biosynthetic process; negative regulation of T cell differentiation in the thymus; negative regulation of transcription from RNA polymerase II promoter; pattern specification process; humoral immune response; negative regulation of humoral immune response mediated by circulating immunoglobulin; negative regulation of T cell proliferation; inhibition of NF-kappaB transcription factor; cilium biogenesis; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; brain development; actin cytoskeleton organization and biogenesis; leukocyte migration; central tolerance induction

Disease: Allergic Rhinitis

Research Articles on HFH4

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Product Notes

The HFH4 foxj1 (Catalog #AAA3015338) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HFH4 Polyclonal Antibody reacts with Human, Mouse, Rat, Dog, Cow, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's HFH4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM). WB: 1:500-2000 IHC-P: 1:100-500 FC/FACS/FCM: 0.2ug/Test. Researchers should empirically determine the suitability of the HFH4 foxj1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HFH4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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