Rabbit anti-Human HFE Protein Polyclonal Antibody | anti-HFE antibody
Anti-HFE Protein (HLA-H)
Gene Names
HFE; HH; HFE1; HLA-H; MVCD7; MGC103790; dJ221C16.10.1
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
HFE Protein; Polyclonal Antibody; Anti-HFE Protein (HLA-H); HLA-H; anti-HFE antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
The human sequence is 100% conserved in human delE3 variant HFE (256 aa); 562-878 variant (242-aa); delE4 variant (334-aa), del#3-7 variant (161 aa), 495-2314del variants (145-aa) and delE3, intron3ins, intron4ins variant (175-aaa). Antibody reactivity with all human HFE variants or HFE from other species has not been studied. We recommend the use for mouse/rat HFE. Control peptide, because of the small size (2-3kD), is not recommended for Western. It should be used in ELISA or antibody blocking (use 5-10 ug control peptide per 1 ug of IgG or 1 ul of antiserum) experiments to demonstrate antibody specificity
Purity/Purification
Serum
Form/Format
Supplied as a liquid, neat serum, 40% glycerol.
Applicable Applications for anti-HFE antibody
ELISA; Western Blot
Application Notes
ELISA: ~1:100,000 using 50-100ng control peptide/well.
Recommended Dilutions: Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for aff. pure IgG using ECL.
Recommended Dilutions: Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for aff. pure IgG using ECL.
Immunogen
A 15-aa peptide sequence within the N-terminus of human HFE (1) was synthesized, coupled to KLH
Source Information
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Temperature: Blue Ice
Shipping Temperature: Blue Ice
Related Product Information for anti-HFE antibody
Elemental iron is required for a variety of normal cellular functions and vital for proper growth and development. However, natural iron is quite insoluble and excess iron is harmful, since it can catalyze the formation of potentially damaging reactive oxygen species. Humans also have very limited capacity to excrete iron. Therefore, cells have developed mechanisms to improve solubility of iron and to control intracellular iron levels at the point of absorption in the intestine and other tissue. Several proteins including Ferritin, transferrin (Tf), transferrin receptors (TfRs), and iron regulatory proteins (IRPs), iron transporter (NRMAP2/DMT1/DCT1) etc play a key role in iron metabolism. Some genes involved in iron-metabolism are associated with genetic disorders such as Friedreich's Ataxia (Frataxin), genetic hemochromatosis (HFE), and Sex-linked anemia (Hephaestin).
Hereditary hemochromatosis (HHC) is most common autosomal recessive disorder characterized by defective intestinal iron absorption, which lead to iron-overload in many tissues and toxic effects. The candidate gene for HHC encodes the HFE protein (formerly called HLA-H) resembling the major histocompatibility complex MHC class-1 molecule. HFE protein (mouse 359 aa, rat 360 aa, human 348 aa, ~48kD) is type I membrane protein. It is found in all tissues except brain. HFE protein binds too Tfr and reduces its affinity for iron-loaded Tf. The HFE Cys282-Tyr (C282Y) is homozygous in 83-100% of HHC subjects in the US and North Europe, and Australia. The C282Y mutation results in the loss of a structural disulfide bond in the alpha-3 domain of the protein, which prevents association with beta-2 microglobulin and proper presentation to the surface. Defects in HFE are also a cause of porphyria cutanes tarda (PCT), a disorder characterized by light-sensitive dermatitis and presence of large amounts of uroporphyrin in urine.
Hereditary hemochromatosis (HHC) is most common autosomal recessive disorder characterized by defective intestinal iron absorption, which lead to iron-overload in many tissues and toxic effects. The candidate gene for HHC encodes the HFE protein (formerly called HLA-H) resembling the major histocompatibility complex MHC class-1 molecule. HFE protein (mouse 359 aa, rat 360 aa, human 348 aa, ~48kD) is type I membrane protein. It is found in all tissues except brain. HFE protein binds too Tfr and reduces its affinity for iron-loaded Tf. The HFE Cys282-Tyr (C282Y) is homozygous in 83-100% of HHC subjects in the US and North Europe, and Australia. The C282Y mutation results in the loss of a structural disulfide bond in the alpha-3 domain of the protein, which prevents association with beta-2 microglobulin and proper presentation to the surface. Defects in HFE are also a cause of porphyria cutanes tarda (PCT), a disorder characterized by light-sensitive dermatitis and presence of large amounts of uroporphyrin in urine.
Product Categories/Family for anti-HFE antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
39,684 Da
NCBI Official Full Name
HFE protein
NCBI Official Synonym Full Names
hemochromatosis
NCBI Official Symbol
HFE
NCBI Official Synonym Symbols
HH; HFE1; HLA-H; MVCD7; MGC103790; dJ221C16.10.1
NCBI Protein Information
hereditary hemochromatosis protein; high Fe; OTTHUMP00000215786; OTTHUMP00000215787; OTTHUMP00000215788; OTTHUMP00000215789; OTTHUMP00000215790; OTTHUMP00000215791; OTTHUMP00000215792; OTTHUMP00000215793; OTTHUMP00000215816; OTTHUMP00000215817; OTTHUMP00000215818; MHC class I-like protein HFE; hereditary hemochromatosis protein HLA-H
UniProt Protein Name
HFE protein
Protein Family
UniProt Gene Name
HFE
UniProt Entry Name
Q6B0J5_HUMAN
Similar Products
Product Notes
The HFE hfe (Catalog #AAA630764) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HFE Protein (HLA-H) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HFE Protein can be used in a range of immunoassay formats including, but not limited to, ELISA; Western Blot. ELISA: ~1:100,000 using 50-100ng control peptide/well. Recommended Dilutions: Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for aff. pure IgG using ECL. Researchers should empirically determine the suitability of the HFE hfe for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HFE Protein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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