Rabbit anti-Human, Mouse HEXIM1 Polyclonal Antibody | anti-HEXIM1 antibody

HEXIM1, ID (HEXIM1, CLP1, EDG1, HIS1, MAQ1, Protein HEXIM1, Cardiac lineage protein 1, Estrogen down-regulated gene 1 protein, Hexamethylene bis-acetamide-inducible protein 1, Menage a quatre protein 1) (FITC)

Cat. Num. AAA6306244

• Gene Names: HEXIM1; CLP1; EDG1; HIS1; MAQ1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: HEXIM1; Polyclonal Antibody; ID (HEXIM1; CLP1; EDG1; HIS1; MAQ1; Protein HEXIM1; Cardiac lineage protein 1; Estrogen down-regulated gene 1 protein; Hexamethylene bis-acetamide-inducible protein 1; Menage a quatre protein 1) (FITC); Menage a quatre protein 1; anti-HEXIM1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HEXIM1. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-HEXIM1 antibody: Western Blot (WB), Immunohistochemistry (IHC), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 194-222 in the central region of human HEXIM1, UniProt Accession #O94992. Species sequence homology: bovine and rat
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of mouse liver tissue lysates (35ug/lane) usingMBS646608. This demonstrates that MBS646608 detected the HEXIM1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue usingMBS646608, followed by peroxidase conjugation of the secondary antibody and DAB staining. This demonstrates the use of MBS646608 for immunohistochemistry.)

Related Product Information for anti-HEXIM1 antibody

Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns.

Product Categories/Family for anti-HEXIM1 antibody

Antibodies; Cardiac Markers

References

1. Dow, E.C., et al. J. Cell. Physiol. 224(1):84-93 (2010). 2. Ogba, N., et al. Oncogene 29(25):3639-3649 (2010). 3. Schonichen, A., et al. Biochemistry 49(14):3083-3091(2010). 4. Czudnochowski, N., et al. J. Mol. Biol. 395(1):28-41 (2010). 5. Krueger, B.J., et al. PLoS ONE 5(8), E12335 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 5453682
• NCBI GeneID: 10614
• NCBI Accession #: NP_006451.1
• NCBI GenBank Nucleotide #: NM_006460.2
• UniProt Accession #: O94992; B2R8Y5
• Molecular Weight: 40,623 Da
• NCBI Official Full Name: protein HEXIM1
• NCBI Official Synonym Full Names: hexamethylene bis-acetamide inducible 1
• NCBI Official Symbol: HEXIM1
• NCBI Official Synonym Symbols: CLP1; EDG1; HIS1; MAQ1
• NCBI Protein Information: protein HEXIM1; HMBA-inducible; menage a quatre 1; cardiac lineage protein 1; menage a quatre protein 1; estrogen down-regulated gene 1 protein; hexamthylene bis-acetamide inducible 1; hexamethylene bisacetamide-inducible protein; hexamethylene bis-acetam
• UniProt Protein Name: Protein HEXIM1
• Protein Family: Protein
• UniProt Gene Name: HEXIM1
• UniProt Synonym Gene Names: CLP1; EDG1; HIS1; MAQ1
• UniProt Entry Name: HEXI1_HUMAN

NCBI Description

Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns. [provided by RefSeq, Jul 2008]

Uniprot Description

HEXIM1: Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Homooligomer and heterooligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA. Up-regulated by HMBA (hexamethylene bisacetamide). Down-regulated by estrogen. Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues. Belongs to the HEXIM family.

Protein type: Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; snRNA binding

Biological Process: transcription, DNA-dependent; heart development; negative regulation of cyclin-dependent protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent