Rabbit anti-Human HER3 (pY1222) Polyclonal Antibody | anti-ERBB3 antibody

HER3 (pY1222) Antibody

Cat. Num. AAA9216518

• Gene Names: ERBB3; HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3
• Reactivity: Human
• Applications: Western Blot
• Synonyms: HER3 (pY1222); Polyclonal Antibody; HER3 (pY1222) Antibody; Purified Rabbit Polyclonal Antibody (Pab); anti-ERBB3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Form/Format: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Sequence Length: 183

• Applicable Applications for anti-ERBB3 antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:1000
• Antigen Source: Human
• Antigen Type: A Synthesized Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-HER3 (pY1222) Antibody at 1:1000 dilution + SK-BR-3 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 148 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-ERBB3 antibody

Binds and is activated by neuregulins and NTAK. May also be activated by CSPG5.

Product Categories/Family for anti-ERBB3 antibody

Cancer; Cell Biology; Developmental Biology; Signal Transduction

References

Kraus M.H.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:9193-9197(1989). Plowman G.D.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:4905-4909(1990). Katoh M.,et al.Biochem. Biophys. Res. Commun. 192:1189-1197(1993). Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 54792102
• NCBI GeneID: 2065
• NCBI Accession #: NP_001005915.1
• NCBI GenBank Nucleotide #: NM_001005915.1
• UniProt Accession #: P21860; Q9BUD7; A8K6L6; B4DIK7; B4DV32; E9PDT8
• Molecular Weight: 148098
• NCBI Official Full Name: receptor tyrosine-protein kinase erbB-3 isoform s
• NCBI Official Synonym Full Names: erb-b2 receptor tyrosine kinase 3
• NCBI Official Symbol: ERBB3
• NCBI Official Synonym Symbols: HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-3
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-3
• Protein Family: Receptor tyrosine-protein kinase
• UniProt Gene Name: ERBB3
• UniProt Synonym Gene Names: HER3
• UniProt Entry Name: ERBB3_HUMAN

NCBI Description

This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

HER3: a receptor tyrosine kinase of the EGFR family. Binds and is activated by neuregulins and NTAK. Can form homodimers or ErbB-2/ErbB-3 heterodimers. Kinase domain lacks activity but heterodimerizes with other EGFRs to transduce growth signals. May be required for HER2 activity. Elevated expression in breast and other tumors is indicative of poor outcome. A secreted form is expressed in metastatic prostate cancer Two alternatively spliced isoforms have been described.

Protein type: Protein kinase, TK; Kinase, protein; Membrane protein, integral; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Oncoprotein; TK group; EGFR family

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: extracellular space; integral to plasma membrane; basolateral plasma membrane; apical plasma membrane; plasma membrane; intracellular; lateral plasma membrane; receptor complex

Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; protein homodimerization activity; protein heterodimerization activity; growth factor binding; protein-tyrosine kinase activity; protein tyrosine kinase activator activity; ATP binding

Biological Process: axon guidance; phosphoinositide 3-kinase cascade; activation of MAPKK activity; nerve growth factor receptor signaling pathway; wound healing; heart development; negative regulation of cell adhesion; signal transduction; small GTPase mediated signal transduction; negative regulation of neuron apoptosis; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; MAPKKK cascade; cranial nerve development; peripheral nervous system development; regulation of cell proliferation; negative regulation of signal transduction; Schwann cell differentiation; positive regulation of phosphoinositide 3-kinase cascade; neuron apoptosis; Ras protein signal transduction; insulin receptor signaling pathway; innate immune response; negative regulation of secretion; vascular endothelial growth factor receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Lethal Congenital Contracture Syndrome 2