Rabbit anti-Human heparin cofactor II Polyclonal Antibody | anti-SERPIND1 antibody

Rabbit anti Human heparin cofactor II

Cat. Num. AAA573577

• Gene Names: SERPIND1; HC2; LS2; HCF2; HCII; HLS2; THPH10; D22S673
• Reactivity: Human
• Applications: Precipitation Assays
• Synonyms: heparin cofactor II; Polyclonal Antibody; Rabbit anti Human heparin cofactor II; anti-SERPIND1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Precipitating polyclonal Rabbit antiSerum to Human heparin cofactor II.

• Applicable Applications for anti-SERPIND1 antibody: Precipitation Assays
• Application Notes: In immunoelectrophoresis use 2 ul or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul in a 3 mm diameter center well and 2 ul serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. For use in electroimmunodiffusion use a concentration of 2% antiserum in the gel. Measured by quantitative precipitin analysis. The amount of heparin cofactor II precipitated by 1 ml antiserum is approximately 90 ug/ml.
• Host Note: Heparin cofactor II is a glycoprotein synthesized in the liver and has a molecular weight of 65,000. It is a plasma serine inhibitor of thrombin. Antithrombin III is more slowly inhibited. A hereditary form of inactive heparin cofactor II has been described. Reduced plasma values have been observed in disseminated intravascular coagulopathy and in lever disease. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Form: Delidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water.
• Reactivity Note: The antiSerum does not cross react with any other component of Human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
• Preparation and Storage: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Related Product Information for anti-SERPIND1 antibody

The reactivity of the antiserum is restricted to heparin cofactor II. In bidimensional electrophoresis against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified immunogen. No reaction is obtained with any other plasma protein component or serum. In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion to identify the presence of heparin cofactor II in human plasma or other body fluids or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included.

Product Categories/Family for anti-SERPIND1 antibody

Complement; Coagulation; Haematology

NCBI and Uniprot Product Information

• NCBI GI #: 183910
• NCBI GeneID: 3053
• UniProt Accession #: P05546; Q6IBZ5; B2RAI1; D3DX34
• NCBI Official Full Name: heparin cofactor II
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade D (heparin cofactor), member 1
• NCBI Official Symbol: SERPIND1
• NCBI Official Synonym Symbols: HC2; LS2; HCF2; HCII; HLS2; THPH10; D22S673
• NCBI Protein Information: heparin cofactor 2; serpin D1; leuserpin 2; protease inhibitor leuserpin-2; serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1
• UniProt Protein Name: Heparin cofactor 2
• Protein Family: Heparin cofactor
• UniProt Gene Name: SERPIND1
• UniProt Synonym Gene Names: HCF2; HC-II; HLS2
• UniProt Entry Name: HEP2_HUMAN

NCBI Description

The product encoded by this gene is a serine proteinase inhibitor which rapidly inhibits thrombin in the presence of dermatan sulfate or heparin. The gene contains five exons and four introns. This protein shares homology with antithrombin III and other members of the alpha 1-antitrypsin superfamily. Mutations in this gene are associated with heparin cofactor II deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

SERPIND1: Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner. Defects in SERPIND1 are the cause of thrombophilia due to heparin cofactor 2 deficiency (THPH10). A hemostatic disorder characterized by a tendency to recurrent thrombosis. Belongs to the serpin family.

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: extracellular space; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; heparin binding; endopeptidase inhibitor activity

Biological Process: blood coagulation; chemotaxis

Disease: Heparin Cofactor Ii Deficiency