CD55/DAF Active Protein | CD55 active protein

Human CellExp CD55 / DAF, human recombinant

Cat. Num. AAA844182

• Gene Names: CD55; CR; TC; DAF; CROM
• Applications: SDS-Page
• Purity: >=95% by SDS-PAGE gel
• Synonyms: CD55/DAF; Human CellExp CD55 / DAF; human recombinant; CD55; DAF; CR; CROM; TC; CD55 active protein

• Purity/Purification: >=95% by SDS-PAGE gel
• Form/Format: Lyophilized
• Sequence Length: 381

• Applicable Applications for CD55 active protein: SDS-PAGE
• Source: HEK293 cells (Asp 35 - Ser 353)
• Formulation: Lyophilized from 0.22 um filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization
• Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: <1 EU/ug by LAL method
• Biological Activity: Measured by its binding ability in a functional ELISA. When rhCD55 is coated at 1 ug/ml (100 ul/well), the concentration of rhCD97 that produces 50% of the optimal binding response is found to be approximately 0.15--2.0 ug/ml.
• Results: Measured by its binding ability in a functional ELISA. When rhCD55 is coated at 1 ug/ml (100 ul/well), the concentration of rhCD97 that produces 50% of the optimal binding response is found to be approximately 0.15--2.0 ug/ml.
• Preparation and Storage: Store at -20 degree C. After reconstitution, aliquot and store at -20 degree C and use within 3 months. Avoid repeated freezing and thawing cycles.

Testing Data

Related Product Information for CD55 active protein

Background: CD55, also known as DAF or decay accelerating factor, is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. CD55 /DAF is a 70 kDa membrane protein that regulates the complement system on the cell surface. It prevents the assembly of the C3bBb complex (the C3-convertase of the alternative pathway) or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex. This glycoprotein is broadly distributed among hematopoietic and non-hematopoietic cells. It is a determinant for the Cromer blood group system. CD55 is known to bind CD97 via the first SCR. It also binds physiologically generated C3 convertases with its second and third SCRs. Binding results in an accelerated "decay", or dissociation of active C3 convertases, thus blocking the development of C' attack complexes on non-foreign cells. Finally, viruses and bacteria are also known to utilize multiple SCR sites for infection.

Product Categories/Family for CD55 active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Cytokines; Growth Factors & Hormones; CDsProteins and Enzymes; Cytokines; Growth Factors &

NCBI and Uniprot Product Information

• NCBI GI #: 10835143
• NCBI GeneID: 1604
• NCBI Accession #: NP_000565.1
• NCBI GenBank Nucleotide #: NM_000574.4
• UniProt Accession #: P08174; P09679; P78361; Q14UF2; Q14UF3; Q14UF4; Q14UF5; Q14UF6; B1AP14; D3DT83; D3DT84; E7ER69
• Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 35.8 kDa. The predicted N-terminus is Asp 35. DTT-reduced Protein migrates as 50-65 kDa due to glycosylation.
• NCBI Official Full Name: complement decay-accelerating factor isoform 1 preproprotein
• NCBI Official Synonym Full Names: CD55 molecule, decay accelerating factor for complement (Cromer blood group)
• NCBI Official Symbol: CD55
• NCBI Official Synonym Symbols: CR; TC; DAF; CROM
• NCBI Protein Information: complement decay-accelerating factor
• UniProt Protein Name: Complement decay-accelerating factor
• Protein Family: Complement decay-accelerating factor
• UniProt Gene Name: CD55
• UniProt Synonym Gene Names: CR; DAF
• UniProt Entry Name: DAF_HUMAN

NCBI Description

This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014]

Uniprot Description

CD55: This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Belongs to the receptors of complement activation (RCA) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Cell surface

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: cell surface; integral to plasma membrane; extracellular region; plasma membrane; lipid raft

Molecular Function: viral receptor activity; protein binding; lipid binding

Biological Process: respiratory burst; elevation of cytosolic calcium ion concentration; entry of virus into host cell; negative regulation of complement activation; regulation of complement activation; innate immune response; complement activation, classical pathway; regulation of lipopolysaccharide-mediated signaling pathway

Disease: Blood Group, Cromer System