Goat Heparin Cofactor II Polyclonal Antibody | anti-SERPIND1 antibody

Goat anti-human Heparin Cofactor II, Affinity-Purified IgG

Cat. Num. AAA512095

• Gene Names: SERPIND1; HC2; LS2; HCF2; HCII; HLS2; D22S673
• Purity: Affinity purified IgG.; Vial containing ml of IgG purified by affinity chromatography on immobilized HCII. Total protein is 0.5 mg.
• Synonyms: Heparin Cofactor II; Polyclonal Antibody; Goat anti-human Heparin Cofactor II; Affinity-Purified IgG; human; anti-SERPIND1 antibody

• Host: Goat
• Clonality: Polyclonal
• Specificity: This antibody is specific for HCII as demonstrated by immunoelectrophoresis and ELISA.
• Purity/Purification: Affinity purified IgG.; Vial containing ml of IgG purified by affinity chromatography on immobilized HCII. Total protein is 0.5 mg.
• Form/Format: Affinity-purified IgG (APIgG), clear liquid.
• Concentration: APIgG concentration is mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 13.4. (varies by lot)

• Application Notes: Suitable as a source of enriched antibodies to human HCII.
• Immunogen: Human Heparin Cofactor II purified from plasma.
• Buffer: 10 mM HEPES, pH 7.4, 150 mM NaCl, 50% (v/v) glycerol.
• Neutralizing Activity: Not determined
• Preparation and Storage: Store between -10 and -20 degree C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use.

Related Product Information for anti-SERPIND1 antibody

Heparin Cofactor II (HCII), also known as heparin cofactor A and dermatan sulphate cofactor, is a single chain glycoprotein produced in the liver and circulates in plasma at concentration of 50-100 ug/ml (0.8-1.5 uM). A member of the SERPIN family of proteinase inhibitors, HCII exhibits a relatively narrow specificity and is only known to inhibit the serine proteinases thrombin and chymotrypsin. The inhibitory activity toward thrombin is accelerated up to 1000-fold in the presence of optimal concentrations of heparin. Unlike Antithrombin, thrombin inhibition by HCII is also enhanced by dermatan sulphate. HCII has an apparent molecular weight of 65,600 daltons and interaction with thrombin results in a covalent 1:1 enzyme-inhibitor complex of 102,600 daltons 1-3.

References

1. Tollefsen DM, Majerus DW, Blank MK; Heparin Cofactor II; JBC 257:5, p2162-2169, 1982.
2. Pratt CW, Whinna HC, Mead JB, Treanor RE, Church FC: Physicochemical aspects of Heparin Cofactor II; Ann N.Y. Acad. Sci.: 556, p104-115, 1989.
3. Griffith MJ, Noyes CM, Church FC; Reactive Site Peptide Structural Similarity between Heparin Cofactor II and Antithrombin III; JBC:260, pp 2218-2225, 1985.

NCBI and Uniprot Product Information

• NCBI GI #: 73858566
• NCBI GeneID: 3053
• NCBI Accession #: NP_000176.2
• NCBI GenBank Nucleotide #: NM_000185.3
• UniProt Accession #: P05546; Q6IBZ5; B2RAI1; D3DX34; Q8IVC0
• Molecular Weight: 54960 Da
• NCBI Official Full Name: heparin cofactor 2
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade D (heparin cofactor), member 1
• NCBI Official Symbol: SERPIND1
• NCBI Official Synonym Symbols: HC2; LS2; HCF2; HCII; HLS2; D22S673
• NCBI Protein Information: heparin cofactor 2; serpin D1; leuserpin 2; OTTHUMP00000198450; OTTHUMP00000198451; protease inhibitor leuserpin-2; serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1
• UniProt Protein Name: Heparin cofactor 2
• Protein Family: Heparin cofactor
• UniProt Gene Name: SERPIND1
• UniProt Synonym Gene Names: HCF2
• UniProt Entry Name: HEP2_HUMAN

NCBI Description

The product encoded by this gene is a serine proteinase inhibitor which rapidly inhibits thrombin in the presence of dermatan sulfate or heparin. The gene contains five exons and four introns. This protein shares homology with antithrombin III and other members of the alpha 1-antitrypsin superfamily. Mutations in this gene are associated with heparin cofactor II deficiency. [provided by RefSeq]

Uniprot Description

Function: Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner. Ref.11Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils. Ref.11

Tissue specificity: Expressed predominantly in liver. Also present in plasma. Ref.15

Domain: The N-terminal acidic repeat region mediates, in part, the glycosaminoglycan-accelerated thrombin inhibition. Ref.11

Post-translational modification: Phosphorylation sites are present in the extracelllular medium.

Involvement in disease: Defects in SERPIND1 are the cause of heparin cofactor 2 deficiency (HCF2D) [

MIM:612356]. HCF2D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. Ref.16 Ref.17 Ref.19 Ref.20

Sequence similarities: Belongs to the serpin family.

Sequence caution: The sequence CAG30459.1 differs from that shown. Reason: Erroneous initiation.