Testing Data
(Formalin-fixed, paraffin-embedded human stomach stained with Helicobacter pylori Rabbit PAb.)
Rabbit Helicobacter Pylori Polyclonal Antibody
Helicobacter Pylori
Reactivity
Helicobacter pylori
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Synonyms
Helicobacter Pylori; Polyclonal Antibody; H. pylori; anti-Helicobacter Pylori antibody
Host
Rabbit
Reactivity
Helicobacter pylori
Clonality
Polyclonal
Isotype
IgG
Specificity
The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.
Form/Format
200ug/ml of Ab Purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Applicable Applications for anti-Helicobacter Pylori antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistology (IH)
Application Notes
Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistology (formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.
Localization
Cell surface and cytoplasm
Positive Control
Helicobacter pylori infected stomach biopsy
Immunogen
Total sonicate of Helicobacter pylori
Preparation and Storage
Store at 2 to 8 degree C. Antibody is stable for 24 months.
Testing Data
(Formalin-fixed, paraffin-embedded human stomach stained with Helicobacter pylori Rabbit PAb.)
Testing Data
(Formalin-fixed, paraffin-embedded human stomach stained with Helicobacter pylori Rabbit PAb.)
References
1. Fiorentino M et. al. Infect Immun 81:876-883 (2013).
2. Moodley Y et. al. PLoS Pathog 8(5): e1002693. doi:10.1371/journal.ppat.1002693 (2012).
2. Moodley Y et. al. PLoS Pathog 8(5): e1002693. doi:10.1371/journal.ppat.1002693 (2012).
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Product Notes
The Helicobacter Pylori (Catalog #AAA438144) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Helicobacter Pylori reacts with Helicobacter pylori and may cross-react with other species as described in the data sheet. AAA Biotech's Helicobacter Pylori can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistology (IH). Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistology (formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. Researchers should empirically determine the suitability of the Helicobacter Pylori for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Helicobacter Pylori, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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