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Western Blot (WB) (Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human K562 Cells; Lane3: Human jurkat Cells.)

Rabbit anti-Human Helicase Like Transcription Factor Polyclonal Antibody | anti-HLTF antibody

Polyclonal Antibody to Helicase Like Transcription Factor (HLTF)

Gene Names
HLTF; ZBU1; HLTF1; RNF80; HIP116; SNF2L3; HIP116A; SMARCA3
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Helicase Like Transcription Factor; Polyclonal Antibody; Polyclonal Antibody to Helicase Like Transcription Factor (HLTF); anti-HLTF antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against HLTF. It has beenselected for its ability to recognize HLTF in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-HLTF antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 1-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Fragment
HLTF (Ala837~Lys996)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human
Conjugate
No Conjugate
Immunogen
Recombinant HLTF (Ala837~Lys996) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2066432
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human K562 Cells; Lane3: Human jurkat Cells.)

Western Blot (WB) (Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human K562 Cells; Lane3: Human jurkat Cells.)

Western Blot (WB)

(Western Blot: Sample: Recombinant HLTF, Human.)

Western Blot (WB) (Western Blot: Sample: Recombinant HLTF, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Liver Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Liver Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Brain Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Brain Tissue.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
99,919 Da
NCBI Official Full Name
helicase-like transcription factor isoform 2
NCBI Official Synonym Full Names
helicase like transcription factor
NCBI Official Symbol
HLTF
NCBI Official Synonym Symbols
ZBU1; HLTF1; RNF80; HIP116; SNF2L3; HIP116A; SMARCA3
NCBI Protein Information
helicase-like transcription factor
UniProt Protein Name
Helicase-like transcription factor
UniProt Gene Name
HLTF
UniProt Synonym Gene Names
HIP116A; RNF80; SMARCA3; SNF2L3; ZBU1

NCBI Description

This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. [provided by RefSeq, Jul 2008]

Uniprot Description

SMARCA3: an enzyme with apparent ATP-dependent DNA helicase activity. Belongs to the SNF2/RAD54 helicase family, RAD16 subfamily. Two alternatively spliced human isoforms have been described.

Protein type: DNA-binding; EC 3.6.1.-; EC 3.6.4.-; EC 6.3.2.-; EC 6.3.2.19; Helicase; Ligase; Nucleolus; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: cytoplasm; membrane; nucleolus; nucleoplasm; nucleus

Molecular Function: ATP binding; ATPase activity; DNA binding; helicase activity; protein binding; RNA binding; ubiquitin protein ligase binding; zinc ion binding

Biological Process: covalent chromatin modification; positive regulation of transcription from RNA polymerase II promoter; protein ubiquitination; regulation of transcription, DNA-templated; transcription from RNA polymerase II promoter

Research Articles on HLTF

Similar Products

Product Notes

The HLTF hltf (Catalog #AAA2026761) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Helicase Like Transcription Factor (HLTF) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Helicase Like Transcription Factor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1-5ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the HLTF hltf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Helicase Like Transcription Factor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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