Western Blot (WB)
(Western blot analysis of extracts from rat brain, using HECTD1 Antibody.)
Rabbit HECTD1 Polyclonal Antibody | anti-HECTD1 antibody
HECTD1 Antibody
Gene Names
HECTD1; EULIR
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, ELISA
Purity
Peptide affinity purification
Synonyms
HECTD1; Polyclonal Antibody; HECTD1 Antibody; E3 ligase for inhibin receptor; E3 ubiquitin protein ligase HECTD1; E3 ubiquitin-protein ligase HECTD1; EULIR; HECD1_HUMAN; HECT domain containing protein 1; HECT domain-containing protein 1; KIAA1131; anti-HECTD1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Clonality
Polyclonal
Isotype
IgG
Specificity
HECTD1 antibody detects endogenous levels of total HECTD1
Purity/Purification
Peptide affinity purification
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
2610
Applicable Applications for anti-HECTD1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000 - 3000
IHC: 1:50 - 1:200
ELISA(peptide): 1:20000 - 1:40000
The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Abin 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
IHC: 1:50 - 1:200
ELISA(peptide): 1:20000 - 1:40000
The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Abin 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human HECTD1, corresponding to a region within the internal amino acids.
Conjugation
Unconjugated
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from rat brain, using HECTD1 Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from rat brain, using HECTD1 Antibody.)
Immunohistochemistry (IHC)
(MBS9609795 at 1/100 staining Human kidney cancer and adjacentnormal tissues by IHC-P. The sample was formaldehyde fixedand a heat mediated antigen retrieval step in citrate bufferwas performed. The sample was then blocked and incubatedwith the primary antibody at 4°C overnight. An HRPconjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(MBS9609795 at 1/100 staining Human kidney cancer and adjacentnormal tissues by IHC-P. The sample was formaldehyde fixedand a heat mediated antigen retrieval step in citrate bufferwas performed. The sample was then blocked and incubatedwith the primary antibody at 4°C overnight. An HRPconjugated anti-Rabbit antibody was used as the secondary antibody.)
Related Product Information for anti-HECTD1 antibody
Function: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates 'Lys-63'-linked polyubiquitination of HSP90AA1 which leads to its intracellular localization and reduced secretion. Negatively regulating HSP90AA1 secretion in cranial mesenchyme cells may impair their emigration and may be essential for the correct development of the cranial neural folds and neural tube closure.
Subunit Structure: Interacts with IGSF1 (PubMed:12421765). Interacts (via N-terminus) with HSP90AA1 (By similarity).
Similarity: Belongs to the UPL family. K-HECT subfamily.
Subunit Structure: Interacts with IGSF1 (PubMed:12421765). Interacts (via N-terminus) with HSP90AA1 (By similarity).
Similarity: Belongs to the UPL family. K-HECT subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
289 kDa
NCBI Official Full Name
E3 ubiquitin-protein ligase HECTD1
NCBI Official Synonym Full Names
HECT domain E3 ubiquitin protein ligase 1
NCBI Official Symbol
HECTD1
NCBI Official Synonym Symbols
EULIR
NCBI Protein Information
E3 ubiquitin-protein ligase HECTD1
UniProt Protein Name
E3 ubiquitin-protein ligase HECTD1
Protein Family
UniProt Gene Name
HECTD1
UniProt Synonym Gene Names
KIAA1131
Uniprot Description
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates 'Lys-63'-linked polyubiquitination of HSP90AA1 which leads to its intracellular localization and reduced secretion. Negatively regulating HSP90AA1 secretion in cranial mesenchyme cells may impair their emigration and may be essential for the correct development of the cranial neural folds and neural tube closure.
Research Articles on HECTD1
Similar Products
Product Notes
The HECTD1 hectd1 (Catalog #AAA9609795) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HECTD1 Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's HECTD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000 - 3000 IHC: 1:50 - 1:200 ELISA(peptide): 1:20000 - 1:40000 The optimal dilutions should be determined by the end user. IMPORTANT: For western blot, incubate membrane with diluted primary Abin 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the HECTD1 hectd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HECTD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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