Three Prime Repair Exonuclease 1 Recombinant Protein | TREX1 recombinant protein

Recombinant Three Prime Repair Exonuclease 1 (TREX1)

Cat. Num. AAA2033284

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Three Prime Repair Exonuclease 1; Recombinant Three Prime Repair Exonuclease 1 (TREX1); AGS1; ATRIP; DRN3; Aicardi-Goutieres Syndrome 1; 3'-5' exonuclease TREX1; DNase III; TREX1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: RRQGR IVQGRPEMCF CPPPTPLPPL RILTLGTHTP TPCSSPGSAA GTYPTMGSQA LPPGPMQTLI FFDMEATGLP FSQPKVTELC LLAVHRCALE SPPTSQGPPP TVPPPPRVVD KLSLCVAPGK ACSPAASEIT GLSTAVLAAH GRQCFDDNLA NLLLAFLRRQ PQPWCLVAHN GDRYDFPLLQ AELAMLGLTS ALDGAFCVDS ITALKALERA SSPSEHGPRK SYSLGSIYTR LYGQSPPDSH TAEGDVLALL SICQWRPQAL LRWVDAHARP FGTIRPMYGV TASARTKPRP SAVTTTAHLA TTRNTSPSLG ESRGTKDLPP VKDPGALSRE GLLAPLGLLA ILTLAVATLYGLSLATPGE
• Sequence Length: 265

• Applicable Applications for TREX1 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 42.2kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Arg6~Glu369
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 646717835
• UniProt Accession #: Q91XB0; Q3TAD7; Q9D6W2; Q9R1B0
• Molecular Weight: 33,675 Da
• NCBI Official Full Name: Three prime repair exonuclease 1
• UniProt Protein Name: Three-prime repair exonuclease 1
• Protein Family: Three-prime repair exonuclease
• UniProt Gene Name: Trex1
• UniProt Entry Name: TREX1_MOUSE

Uniprot Description

TREX1: the major 3' DNA exonuclease in mammalian cells. Normally associates with the endoplasmic reticulum (ER). Translocates to the nucleus at S phase after DNA damage by gamma-irradiation or hydroxyurea. Trex1-deficient cells show defective G1/S transition, with single-stranded DNA molecules persisting after S phase and accumulating in the ER. Degrades ssDNA. Prevents chronic checkpoint activation and inappropriate immune activation. Mutations cause Aicardi-Goutieres syndrome, an autoimmune disorder. Trex1a(-/-) mice have autoinflammatory responses. The gene for this protein is either identical to or adjacent to that of ATRIP. Some of the mRNAs that encode TREX1 also encode ATRIP in another reading frame. Three alternatively spliced human isoforms have been reported.

Protein type: Deoxyribonuclease; DNA repair, damage; Cell cycle regulation; EC 3.1.11.2; DNA-binding

Cellular Component: cytoplasm; endoplasmic reticulum; membrane; nucleus

Molecular Function: 3'-5' exonuclease activity; 3'-5'-exodeoxyribonuclease activity; adenyl deoxyribonucleotide binding; double-stranded DNA binding; exodeoxyribonuclease III activity; exonuclease activity; hydrolase activity; metal ion binding; MutLalpha complex binding; MutSalpha complex binding; nuclease activity; nucleic acid binding; protein binding; protein homodimerization activity; single-stranded DNA binding

Biological Process: DNA metabolic process