Immunohistochemistry (IHC)
(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) stained using MBS618511.)
Rabbit Heat Shock Protein 90, alpha Polyclonal Antibody | anti-HSP90B2P antibody
Heat Shock Protein 90, alpha (HSP90a)
Gene Names
HSP90B2P; HSP; TRAP1; GRP94b; HSPCP2; TRA1P1; GRP94P1; HsGrp94b; HSP90B2P
Reactivity
Chicken, Drosophila, Human, Monkey, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Heat Shock Protein 90; alpha; Polyclonal Antibody; alpha (HSP90a); Anti -Heat Shock Protein 90; anti-HSP90B2P antibody
Host
Rabbit
Reactivity
Chicken, Drosophila, Human, Monkey, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes Human Heat Shock Protein 90a. Species Crossreactivity: Chicken, Drosophila, Monkey, Mouse, Rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Applicable Applications for anti-HSP90B2P antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Immunohistochemistry, ELISA and Western Blot.
Dilution: Immunohistochemistry (Formalin fixed, paraffin): 2.5ug/ml. Validated for use on a panel of 21 formalin-fixed, paraffin embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.
Western Blot: 1:500-1:2500
ELISA: 1:5000-1:25,000
Dilution: Immunohistochemistry (Formalin fixed, paraffin): 2.5ug/ml. Validated for use on a panel of 21 formalin-fixed, paraffin embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.
Western Blot: 1:500-1:2500
ELISA: 1:5000-1:25,000
Immunogen
Synthetic peptide corresponding to aa289-300 from human Heat Shock Protein 90, alpha (KLH)
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) stained using MBS618511.)
Immunohistochemistry (IHC)
(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) stained using MBS618511.)
Related Product Information for anti-HSP90B2P antibody
Hsp90 is a member of the heat shock protein 90 family that functions as a molecular chaperone and has ATPase activity. Hsp90 family proteins are highly conserved between isoforms and species. Several signal transduction pathways depend on Hsp90 function including erbB2, steroid hormone receptors (such as androgen, progesterone, glucocorticoid, and aryl-hydrocarbon), and hypoxia sensing (Hif1alpha). Recent reports show that tumor cells are more sensitive to Hsp90 inhibition and that Hsp90 from tumor cells is more sensitive to small molecule inhibitors (eg 17AAG). The mechanism of this differential sensitivity of normal versus tumor Hsp90 is not known (although mutation has been ruled out). One possible mechanism may be differences in post-translational modification of tumor Hsp90. Hsp90 is a cytoplasmic protein that forms a homodimer in vivo, and interacts with TOM34, AHSA1, HDAC6 and SMYD3.
Product Categories/Family for anti-HSP90B2P antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
45,859 Da
NCBI Official Full Name
heat shock protein 94b
NCBI Official Synonym Full Names
heat shock protein 90kDa beta (Grp94), member 2 (pseudogene)
NCBI Official Symbol
HSP90B2P
NCBI Official Synonym Symbols
HSP; TRAP1; GRP94b; HSPCP2; TRA1P1; GRP94P1; HsGrp94b; HSP90B2P
UniProt Protein Name
Putative endoplasmin-like protein
Protein Family
UniProt Gene Name
HSP90B2P
UniProt Synonym Gene Names
GRP94B; GRP94P1; TRAP1
UniProt Entry Name
ENPLL_HUMAN
Uniprot Description
HSP90B2P: Putative molecular chaperone. Belongs to the heat shock protein 90 family.
Protein type: Heat shock protein
Chromosomal Location of Human Ortholog: 15q26.3
Molecular Function: unfolded protein binding; ATP binding
Biological Process: protein folding; response to stress
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Product Notes
The HSP90B2P hsp90b2p (Catalog #AAA618511) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Heat Shock Protein 90, alpha (HSP90a) reacts with Chicken, Drosophila, Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Heat Shock Protein 90, alpha can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Immunohistochemistry, ELISA and Western Blot. Dilution: Immunohistochemistry (Formalin fixed, paraffin): 2.5ug/ml. Validated for use on a panel of 21 formalin-fixed, paraffin embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. Western Blot: 1:500-1:2500 ELISA: 1:5000-1:25,000. Researchers should empirically determine the suitability of the HSP90B2P hsp90b2p for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heat Shock Protein 90, alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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