Western Blot (WB)
(Western Blot: Sample: Rat Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat HSPA8 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Rat Heat Shock 70kDa Protein 8 (HSPA8) Polyclonal Antibody | anti-HSPA8 antibody
Polyclonal Antibody to Heat Shock 70kDa Protein 8 (HSPA8)
MGHHHHHHSGSEF-DKVSSKN SLESYAFNMK ATVEDEKLQG KINDEDKQKI LDKCNEIISW LDKNQTAEKE EFEHQQKELE KVCNPIITKL YQSAG
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Rat Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat HSPA8 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Rat Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat HSPA8 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded lung tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded lung tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Small intestine Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Small intestine Tissue))
NCBI and Uniprot Product Information
NCBI Description
member of the hsp70 protein; expressed in unstressed cells [RGD, Feb 2006]
Uniprot Description
HSC70: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co- chaperones. Interacts with HSPH1/HSP105. Interacts with IRAK1BP1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with PACRG and TSC2. Interacts with BAG1. Interacts with SV40 VP1. Interacts with DNAJC7. Interacts with HERC5. Interacts with CITED1 (via N-terminus); the interaction suppresses the association of CITED1 to p300/CBP and Smad-mediated transcription transactivation. Constitutively synthesized. Ubiquitous. Belongs to the heat shock protein 70 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Chaperone; Heat shock protein; Nucleolus; RNA-binding
Chromosomal Location of Human Ortholog: 8q22
Cellular Component: asymmetric synapse; autophagic vacuole; axon; cell soma; cell surface; cytoplasm; cytosol; dendrite; dendritic shaft; dendritic spine; extracellular matrix; extracellular space; focal adhesion; intermediate filament; intracellular; intracellular ribonucleoprotein complex; late endosome; lysosomal membrane; lysosome; melanosome; membrane; membrane raft; microtubule; myelin sheath; neuron projection; nucleolus; nucleus; perikaryon; perinuclear region of cytoplasm; photoreceptor inner segment; plasma membrane; postsynaptic density; protein complex; spliceosomal complex; synaptic vesicle; terminal bouton; ubiquitin ligase complex
Molecular Function: A1 adenosine receptor binding; ADP binding; ATP binding; ATPase activity; ATPase activity, coupled; cadherin binding; enzyme binding; G-protein coupled receptor binding; heat shock protein binding; peptide binding; phosphatidylserine binding; protein binding; receptor binding; RNA binding; transcription factor binding; ubiquitin protein ligase binding; unfolded protein binding
Biological Process: aging; ATP metabolic process; axon cargo transport; cellular protein complex disassembly; cerebellum development; chaperone cofactor-dependent protein folding; forebrain development; G1/S transition of mitotic cell cycle; kidney development; mRNA processing; negative regulation of transcription, DNA-dependent; positive regulation of catalytic activity; positive regulation of mRNA splicing, via spliceosome; positive regulation of phagocytosis; positive regulation of proteolysis; positive regulation of T cell mediated cytotoxicity; protein autophosphorylation; protein folding; protein import into nucleus; protein refolding; regulation of cell cycle; regulation of protein stability; response to activity; response to drug; response to estradiol; response to ethanol; response to heat; response to nickel ion; response to progesterone; response to starvation; RNA splicing; sensory perception of smell; skeletal muscle tissue development; synaptic vesicle uncoating; transcription, DNA-dependent
Research Articles on HSPA8
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Product Notes
The HSPA8 hspa8 (Catalog #AAA2006716) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Heat Shock 70kDa Protein 8 (HSPA8) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Heat Shock 70kDa Protein 8 (HSPA8) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the HSPA8 hspa8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSG SEF-DKVSSK N SLESYAFNMK ATVEDEKLQG KINDEDKQKI LDKCNEIISW LDKNQTAEKE EFEHQQKELE KVCNPIITKL YQSAG. It is sometimes possible for the material contained within the vial of "Heat Shock 70kDa Protein 8 (HSPA8), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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