Western Blot (WB)
(Both anti-HDAC9 N-term and C-term Pab were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells. Top figure shows both Pab specifically detect HDAC9 in HeLa-HDAC9 transfected cell but not HeLa alone.)
Rabbit anti-Human HDAC9 Polyclonal Antibody | anti-HDAC9 antibody
HDAC9, NT (HDAC9, HDAC7, HDAC7B, HDRP, KIAA0744, MITR, Histone deacetylase 9, Histone deacetylase 7B, Histone deacetylase-related protein, MEF2-interacting transcription repressor MITR) (Biotin)
ELISA: 1:1,000
IHC: 1:50-100
IP: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Western Blot (WB)
(Both anti-HDAC9 N-term and C-term Pab were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells. Top figure shows both Pab specifically detect HDAC9 in HeLa-HDAC9 transfected cell but not HeLa alone.)
Western Blot (WB)
(Both anti-HDAC9 N-term and C-term Pab were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells. Top figure shows both Pab specifically detect HDAC9 in HeLa-HDAC9 transfected cell but not HeLa alone.)
Immunoprecipitation (IP)
(This figure shows that both Pab can immunoprecipitate (IP) HDAC9 from HeLa-HDAC9 tranfected cells. (Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL).)
Immunoprecipitation (IP)
(This figure shows that both Pab can immunoprecipitate (IP) HDAC9 from HeLa-HDAC9 tranfected cells. (Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL).)
Immunoblot (IB)
(Figure 1: Immunoblots for MITR (AP1109a HDAC9 N-term antibody), Mirk, MyoD and tubulin proteins are shown for cytoplasmic (Cyt) and nuclear (N) extracts from undifferentiated C2C12 myoblasts. Before cell collection for fractionation, the cells are transfected with plasmids coding for Mirk (Wt), kinase-inactive Mirk (YF) or MITR. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.)
Immunoblot (IB)
(Figure 1: Immunoblots for MITR (AP1109a HDAC9 N-term antibody), Mirk, MyoD and tubulin proteins are shown for cytoplasmic (Cyt) and nuclear (N) extracts from undifferentiated C2C12 myoblasts. Before cell collection for fractionation, the cells are transfected with plasmids coding for Mirk (Wt), kinase-inactive Mirk (YF) or MITR. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.)
Immunofluorescence (IF)
(Figure 2: Immunofluorescence staining of MITR for a compartmentalization study in undifferentiated C2C12 myoblasts transfected with a MITR-expressing plasmid. MITR is detected by using the HDAC9 N-term antibody (top panel) or a DYKDDDDK antibody (bottom panel) detecting a DYKDDDDK epitope fused at the N-term end of the MITR construct. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.)
Immunofluorescence (IF)
(Figure 2: Immunofluorescence staining of MITR for a compartmentalization study in undifferentiated C2C12 myoblasts transfected with a MITR-expressing plasmid. MITR is detected by using the HDAC9 N-term antibody (top panel) or a DYKDDDDK antibody (bottom panel) detecting a DYKDDDDK epitope fused at the N-term end of the MITR construct. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.)
Immunofluorescence (IF)
(Figure 2: Immunofluorescence staining of MITR for a compartmentalization study in undifferentiated C2C12 myoblasts transfected with a MITR-expressing plasmid. MITR is detected by using the HDAC9 N-term antibody (top panel) or a DYKDDDDK antibody (bottom panel) detecting a DYKDDDDK epitope fused at the N-term end of the MITR construct. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.)
Immunofluorescence (IF)
(Figure 2: Immunofluorescence staining of MITR for a compartmentalization study in undifferentiated C2C12 myoblasts transfected with a MITR-expressing plasmid. MITR is detected by using the HDAC9 N-term antibody (top panel) or a DYKDDDDK antibody (bottom panel) detecting a DYKDDDDK epitope fused at the N-term end of the MITR construct. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.)
NCBI and Uniprot Product Information
NCBI Description
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
HDAC9: a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Protein type: Transcription, coactivator/corepressor; Deacetylase; EC 3.5.1.98
Chromosomal Location of Human Ortholog: 7p21.1
Cellular Component: nucleoplasm; histone methyltransferase complex; transcription factor complex; histone deacetylase complex; cytoplasm; nucleus
Molecular Function: protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); protein kinase C binding; NAD-dependent histone deacetylase activity (H3-K14 specific); histone deacetylase binding; metal ion binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription corepressor activity; transcription factor binding
Biological Process: Notch signaling pathway; regulation of skeletal muscle fiber development; B cell activation; transcription, DNA-dependent; cellular response to insulin stimulus; regulation of striated muscle cell differentiation; B cell differentiation; heart development; histone deacetylation; negative regulation of transcription from RNA polymerase II promoter; inflammatory response; negative regulation of transcription, DNA-dependent
Research Articles on HDAC9
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Product Notes
The HDAC9 hdac9 (Catalog #AAA6305946) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HDAC9, NT (HDAC9, HDAC7, HDAC7B, HDRP, KIAA0744, MITR, Histone deacetylase 9, Histone deacetylase 7B, Histone deacetylase-related protein, MEF2-interacting transcription repressor MITR) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HDAC9 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). IF: 1:1,000 ELISA: 1:1,000 IHC: 1:50-100 IP: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HDAC9 hdac9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDAC9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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