Rabbit anti-Human HDAC8 Polyclonal Antibody | anti-HDAC8 antibody

HDAC8 (Phospho-Ser39) Antibody

Cat. Num. AAA9400985

• Gene Names: HDAC8; HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: HDAC8; Polyclonal Antibody; HDAC8 (Phospho-Ser39) Antibody; HD8; HDA8; HDACL1; anti-HDAC8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of HDAC8 only when phosphorylated at serine 39.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 286

• Applicable Applications for anti-HDAC8 antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Immunohistochemistry: 1:50~1:100; Immunofluorescence: 1:100~1:200
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of serine 39 (R-A-S(p)-M-V) derived from Human HDAC8.
• Modification: Phospho-Ser39
• Target Name: HDAC8
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using HDAC8(Phospho-Ser39) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells using HDAC8(Phospho-Ser39) Antibody.)

Related Product Information for anti-HDAC8 antibody

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by HDAC8 gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-HDAC8 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 262073088
• NCBI GeneID: 55869
• NCBI Accession #: NP_001159890.1
• NCBI GenBank Nucleotide #: NM_001166418.1
• UniProt Accession #: Q9BY41; Q86VC8; Q9NP76; Q9NYH4; A6ND12; A6ND61; A6NET3; A6NJR3; A8MQ62; B4DKN0; B4DV22
• Molecular Weight: 16,050 Da
• NCBI Official Full Name: histone deacetylase 8 isoform 2
• NCBI Official Synonym Full Names: histone deacetylase 8
• NCBI Official Symbol: HDAC8
• NCBI Official Synonym Symbols: HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1
• NCBI Protein Information: histone deacetylase 8
• UniProt Protein Name: Histone deacetylase 8
• UniProt Gene Name: HDAC8
• UniProt Synonym Gene Names: HDACL1; HD8
• UniProt Entry Name: HDAC8_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

HDAC8: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility. Belongs to the histone deacetylase family. HD type 1 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.5.1.98

Chromosomal Location of Human Ortholog: Xq13

Cellular Component: nucleoplasm; nuclear chromosome; histone deacetylase complex; cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent histone deacetylase activity (H3-K14 specific); metal ion binding; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription factor binding

Biological Process: chromatin assembly or disassembly; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; sister chromatid cohesion; mitotic cell cycle; chromatin modification; negative regulation of transcription from RNA polymerase II promoter

Disease: Wilson-turner X-linked Mental Retardation Syndrome