Rabbit anti-Human HDAC7 Polyclonal Antibody | anti-HDAC7 antibody

HDAC7 Antibody

Cat. Num. AAA9404209

• Gene Names: HDAC7; HD7; HD7A; HDAC7A
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Synonyms: HDAC7; Polyclonal Antibody; HDAC7 Antibody; histone deacetylase 7; HD7A; HDAC7A; anti-HDAC7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total HDAC7 protein.
• Form/Format: Supplied at 0.4mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
• Sequence Length: 991

• Applicable Applications for anti-HDAC7 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:1000-1:2000; Western blotting: 1:500-1:2000; Immunohistochemistry: 1:10-1:50
• Immunogen Type: Peptide-KLH
• Immunogen Description: Synthetic peptide corresponding to a region derived from 938-951 amino acids of Human histone deacetylase 7
• Target Name: HDAC7
• Preparation and Storage: Store at -20 degree C/1 year

Testing Data

(Gel: 8%SDS-PAGE Lysate: 30 ug Hepg2 cell lysate. Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1.5 minutes)

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded Human brain cancer tissue using 31217 (HDAC7 Antibody) at dilution 1/10, on the right is treated with the synthetic peptide.)

Related Product Information for anti-HDAC7 antibody

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-HDAC7 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 169234807
• NCBI GeneID: 51564
• NCBI Accession #: NP_056216
• NCBI GenBank Nucleotide #: NM_015401.4
• Molecular Weight: 66,187 Da
• NCBI Official Full Name: histone deacetylase 7 isoform a
• NCBI Official Synonym Full Names: histone deacetylase 7
• NCBI Official Symbol: HDAC7
• NCBI Official Synonym Symbols: HD7; HD7A; HDAC7A
• NCBI Protein Information: histone deacetylase 7
• UniProt Protein Name: Histone deacetylase 7
• UniProt Gene Name: HDAC7
• UniProt Synonym Gene Names: HDAC7A; HD7; HD7a
• UniProt Entry Name: HDAC7_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC7: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene. Interacts with HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, NCOR1, NCOR2, SIN3A, SIN3B, RBBP4, RBBP7, MTA1L1, SAP30 and MBD3. Interacts with the 14-3-3 protein YWHAE, MEF2A, MEF2B and MEF2C. Interacts with KAT5 and EDNRA. Interacts with KDM5B. Interacts with ZMYND15. Belongs to the histone deacetylase family. HD type 2 subfamily. 10 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.1.98; Hydrolase

Chromosomal Location of Human Ortholog: 12q13.1

Cellular Component: cytoplasm; histone deacetylase complex; nucleoplasm; nucleus

Molecular Function: chromatin binding; metal ion binding; NAD-dependent histone deacetylase activity (H3-K14 specific); protein binding; protein kinase binding; protein kinase C binding; transcription activator binding; transcription corepressor activity

Biological Process: cellular protein metabolic process; intercellular junction assembly; negative regulation of interleukin-2 production; negative regulation of osteoblast differentiation; negative regulation of transcription from RNA polymerase II promoter; neuron apoptosis; neuron differentiation; Notch signaling pathway; post-translational protein modification; protein sumoylation; transcription, DNA-dependent; vasculogenesis