Rabbit HDAC6 Polyclonal Antibody | anti-HDAC6 antibody

HDAC6 (Histone Deacetylase 6, FLJ16239, HD6, JM21) (HRP)

Cat. Num. AAA6420087

• Gene Names: HDAC6; HD6; JM21
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: HDAC6; Polyclonal Antibody; HDAC6 (Histone Deacetylase 6; FLJ16239; HD6; JM21) (HRP); anti-HDAC6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes HDAC6. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-HDAC6 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human HDAC6.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using HDAC6 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using HDAC6 antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-HDAC6 antibody

Antibodies; Enzymes; Deacetylase

NCBI and Uniprot Product Information

• NCBI GI #: 13128864
• NCBI GeneID: 10013
• NCBI Accession #: NP_006035.2
• NCBI GenBank Nucleotide #: NM_006044.2
• UniProt Accession #: Q9UBN7; O94975; Q6NT75; Q7L3E5; Q96CY0
• Molecular Weight: 131,419 Da
• NCBI Official Full Name: histone deacetylase 6
• NCBI Official Synonym Full Names: histone deacetylase 6
• NCBI Official Symbol: HDAC6
• NCBI Official Synonym Symbols: HD6; JM21
• NCBI Protein Information: histone deacetylase 6
• UniProt Protein Name: Histone deacetylase 6
• UniProt Gene Name: HDAC6
• UniProt Synonym Gene Names: KIAA0901; HD6
• UniProt Entry Name: HDAC6_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC6: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Interacts with CBFA2T3, HDAC11 and SIRT2. Interacts with F-actin. Interacts with BBIP10. Under proteasome impairment conditions, interacts with UBD via its histone deacetylase 1 and UBP-type zinc-finger regions. Interacts with CYLD. Interacts with ZMYND15. Belongs to the histone deacetylase family. HD type 2 subfamily.

Protein type: Nuclear receptor co-regulator; EC 3.5.1.98; Ubiquitin conjugating system; Deacetylase

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: microtubule; dendrite; histone deacetylase complex; leading edge; perikaryon; caveola; inclusion body; cytosol; nucleoplasm; dynein complex; microtubule associated complex; cytoplasmic microtubule; axon; perinuclear region of cytoplasm; cytoplasm; nucleus

Molecular Function: zinc ion binding; histone deacetylase binding; microtubule binding; beta-tubulin binding; beta-catenin binding; misfolded protein binding; Hsp90 protein binding; actin binding; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); tubulin deacetylase activity; ubiquitin protein ligase binding; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; polyubiquitin binding; tau protein binding; alpha-tubulin binding

Biological Process: negative regulation of proteolysis; response to misfolded protein; protein polyubiquitination; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; positive regulation of signal transduction; transcription, DNA-dependent; regulation of fat cell differentiation; negative regulation of microtubule depolymerization; macroautophagy; organelle organization and biogenesis; response to toxin; misfolded or incompletely synthesized protein catabolic process; histone deacetylation; regulation of gene expression, epigenetic; response to organic substance; intracellular protein transport; protein complex disassembly; protein amino acid deacetylation; lysosome localization; negative regulation of oxidoreductase activity; regulation of receptor activity; negative regulation of transcription, DNA-dependent; negative regulation of protein complex disassembly

Disease: Chondrodysplasia With Platyspondyly, Distinctive Brachydactyly, Hydrocephaly, And Microphthalmia