anti-Human, Mouse HDAC4 Polyclonal Antibody | anti-HDAC4 antibody

Rabbit Polyclonal (IgG) to Human HDAC4

Cat. Num. AAA241836

• Gene Names: HDAC4; HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
• Reactivity: Human, Mouse
• Purity: Protein G Purified
• Synonyms: HDAC4; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human HDAC4; HDAC4 Antibody; BDMR Antibody; AHO3 Antibody; Histone deacetylase A Antibody; HD4 Antibody; HA6116 Antibody; HDAC-A Antibody; HDACA Antibody; Histone deacetylase 4 Antibody; HDAC-4 Antibody; KIAA0288 Antibody; anti-HDAC4 antibody

• Host: HDAC4 antibody was produced in a Rabbit.
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: A 140 kD band is observed.
• Purity/Purification: Protein G Purified
• Form/Format: PBS, 0.05% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1084

• Application Notes: Immunohistochemistry: MBS241836 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS241386 was determined to be 5 ug/ml.; ; IHC - Paraffin (5 µg/ml); Western blot (5 - 2 µg/ml); Immunoprecipitation; Chromatin Immunoprecipitation
• Target: HDAC4
• Epitope: aa194-209
• Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial . We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide . The chemical, physical , and toxicological properties of this material have not been thorughly investigated. Standard Laboratory Practices should be followed. Avoid skin and eye contact, inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plumbing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
• Immunogen: HDAC4 antibody was raised against synthetic peptide from human HDAC4
• Immunogen Species: HDAC4 antibody was raised against human.
• Antigen Type: Synthetic peptide.
• Epitope: aa194-209
• Family/Subfamily: Histone Deacetylase/not assigned-Histone Deacetylase
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short term 4°C,long term aliquot and store at -20°C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-HDAC4 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of HDAC-4 in Jurkat cell lysate with antibody.)

Product Categories/Family for anti-HDAC4 antibody

Family: Histone Deacetylase

NCBI and Uniprot Product Information

• NCBI GI #: 153085395
• NCBI GeneID: 9759
• NCBI Accession #: NP_006028.2
• NCBI GenBank Nucleotide #: NM_006037.3
• UniProt Accession #: P56524; Q86YH7; Q9UND6; E9PGB9; F5GX36
• NCBI Official Full Name: histone deacetylase 4
• NCBI Official Synonym Full Names: histone deacetylase 4
• NCBI Official Symbol: HDAC4
• NCBI Official Synonym Symbols: HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
• NCBI Protein Information: histone deacetylase 4; histone deacetylase A
• UniProt Protein Name: Histone deacetylase 4
• UniProt Gene Name: HDAC4
• UniProt Synonym Gene Names: KIAA0288; HD4
• UniProt Entry Name: HDAC4_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC4: a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events. Does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.

Protein type: EC 3.5.1.98; Nuclear receptor co-regulator; Deacetylase; Hydrolase

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: nucleoplasm; transcriptional repressor complex; histone deacetylase complex; cytoplasm; actomyosin; cytosol; Z disc; nucleus; neuromuscular junction; A band

Molecular Function: potassium ion binding; transcription activator binding; zinc ion binding; histone deacetylase binding; protein deacetylase activity; transcription factor binding; protein kinase binding; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; NAD-dependent histone deacetylase activity (H3-K14 specific); sequence-specific DNA binding; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription corepressor activity

Biological Process: cardiac muscle hypertrophy; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; response to denervation involved in regulation of muscle adaptation; regulation of gene expression, epigenetic; negative regulation of cell proliferation; positive regulation of cell proliferation; negative regulation of osteoblast differentiation; inflammatory response; negative regulation of glycolysis; skeletal development; response to drug; nervous system development; regulation of skeletal muscle fiber development; B cell activation; transcription, DNA-dependent; negative regulation of transcription factor activity; histone deacetylation; osteoblast development; positive regulation of protein sumoylation; chromatin remodeling; regulation of protein binding; B cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of transcription, DNA-dependent