Rabbit anti-Human, Mouse HDAC2 Polyclonal Antibody | anti-HDAC2 antibody

Anti-HDAC2 Antibody

Cat. Num. AAA419973

• Gene Names: HDAC2; HD2; RPD3; YAF1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: HDAC2; Polyclonal Antibody; Anti-HDAC2 Antibody; anti-HDAC2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Western blot analysis of extracts of various cell lines, using HDAC2 antibody.
• Purity/Purification: Affinity Purification
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 488

• Applicable Applications for anti-HDAC2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50- 1:200; IF: 1:20- 1:50
• Immunogen: A synthetic peptide of human HDAC2
• Storage Buffer: PBS with 0.02% sodium azide,50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

Related Product Information for anti-HDAC2 antibody

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1, 2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4,5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6, 7). HDAC1 and HDAC2 are highly homologous and are involved in histone deacetylation, chromatin remodeling and transcriptional repression (8-10). Both proteins are found together in numerous complexes including the nucleosome remodeling and deacetylation complex (NuRD), MeCP1, and the mSin3A corepressor complex.

NCBI and Uniprot Product Information

• NCBI GI #: 293336691
• NCBI GeneID: 3066
• NCBI Accession #: NP_001518.3
• NCBI GenBank Nucleotide #: NM_001527.3
• UniProt Accession #: Q92769; Q5SRI8; Q5SZ86; Q8NEH4; B3KRS5; B4DL58; E1P561
• Molecular Weight: 51,998 Da
• NCBI Official Full Name: histone deacetylase 2
• NCBI Official Synonym Full Names: histone deacetylase 2
• NCBI Official Symbol: HDAC2
• NCBI Official Synonym Symbols: HD2; RPD3; YAF1
• NCBI Protein Information: histone deacetylase 2
• UniProt Protein Name: Histone deacetylase 2
• UniProt Gene Name: HDAC2
• UniProt Synonym Gene Names: HD2

NCBI Description

This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]

Uniprot Description

HDAC2: a transcriptional regulator of the histone deacetylase family, subfamily 1. Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation plays a role in epigenetic repression and transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.

Protein type: Deacetylase; EC 3.5.1.98; Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cytoplasm; ESC/E(Z) complex; nuclear chromatin; nucleoplasm; nucleus; NuRD complex; protein complex; Sin3 complex

Molecular Function: chromatin binding; deacetylase activity; enzyme binding; histone deacetylase activity; NF-kappaB binding; nucleosomal DNA binding; protein binding; protein deacetylase activity; sequence-specific DNA binding; transcription factor binding

Biological Process: ATP-dependent chromatin remodeling; blood coagulation; chromatin remodeling; circadian regulation of gene expression; dendrite development; embryonic digit morphogenesis; epidermal cell differentiation; histone deacetylation; maintenance of chromatin silencing; negative regulation of apoptosis; negative regulation of MHC class II biosynthetic process; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; odontogenesis of dentine-containing teeth; positive regulation of cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of proteolysis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent