HDAC1 Polyclonal Antibody | anti-HDAC1 antibody

Anti-HDAC1 Rabbit pAb

Cat. Num. AAA477133

• Gene Names: HDAC1; HD1; RPD3; GON-10; RPD3L1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified
• Synonyms: HDAC1; Polyclonal Antibody; Anti-HDAC1 Rabbit pAb; DKFZp686H12203; GON 10; HD 1; HD1; HDAC 1; hdac1: histone deacetylase 1; HDAC1_HUMAN; Histone deacetylase 1 (HD1); Histone deacetylase 1; Reduced potassium dependency yeast homolog like 1; RPD3 (reduced potassium dependency yeast homolog) like 1; RPD3 (reduced potassium dependency); RPD3; RPD3L1; anti-HDAC1 antibody

• Host: Rabbit; Source: Human
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: 10mM PBS with 0.02% sodium azide, 50% glycerol.
• Sequence Length: 482

• Applicable Applications for anti-HDAC1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human HDAC1 around the phosphorylation site of serine 421/423.
• Sensitivity: This antibody detects endogenous levels of HDAC1 and does not cross-react with related proteins.
• Sequence Similarities: Belongs to the histone deacetylase family. HD type 1 subfamily.
• Subunit Structure: Interacts with C10orf90/FATS (via its N-terminal): the interaction prevents binding of HDAC1 to CDKN1A/p21 and facilitates the acetylation and stabilization of CDKN1A/p21. Interacts with CDKN1A/p21; the interaction is prevented by binding of C10orf90/FATS facilitating acetylation and stabilization of CDKN1A/p21. Component of a RCOR/GFI/KDM1A/HDAC complex. Interacts directly with GFI1 and GFI1B. Part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, KDM1A, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Associates with the 9-1-1 complex; interacts with HUS1. Found in a complex with DNMT3A and HDAC7. Interacts with BAZ2A/TIP5, BCOR, BRMS1L, DAXX, DNMT1, EP300, HCFC1, NFE4, PCAF, PHB2, MIER1, KDM4A, MINT, NRIP1, PRDM6, RERE, SETDB1, SMYD2, SUV39H1, TGIF, TGIF2, UHRF1, UHRF2 and ZNF541. Interacts with the non-histone region of H2AFY. Interacts with HDAC9. Component of a mSin3A corepressor complex that contains SIN3A, SAP130, SUDS3/SAP45, ARID4B/SAP180, HDAC1 and HDAC2. Interacts with BANP, CBFA2T3 and KDM5B. Interacts with SAP30L. Interacts with E4F1. Interacts with KFL1 and SAMSN1. Interacts with SV40 large T antigen. Interacts with CHFR, PRDM16 and SMAD3. Interacts with SP1; the interaction deacetylates SP1 and regulates its transcriptional activity. Interacts with SP3; the interaction deacetylates SP3 and regulates its transcriptional activity. In vitro, C18 ceramides increase this interaction and the subsequent SP3 deacetylation and SP3-mediated repression of the TERT promoter. Interacts with RB1 and SMARCA4/BRG1. Interacts with TRAF6. Interacts with TSHZ3 (via N-terminus); the interaction is direct. Found in a trimeric complex with APBB1 and TSHZ3; the interaction between HDAC1 and APBB1 is mediated by TSHZ3. Interacts with APEX1; the interaction is not dependent on the acetylated status of APEX1. Interacts with NR4A2/NURR1 and BRMS1. Interacts with TRIM28; the interaction recruits HDAC1 to E2F1 and inhibits its acetylation. Binds to CDK5 complexed to CDK5R1 (p25). Interacts with ZMYND15. Interacts with DDX5. Part of a complex composed of TRIM28, HDAC1, HDAC2 and EHMT2. Interacts with BCL6 and DDIT3/CHOP. Part of a complex containing at least CDYL, MIER1, MIER2, HDAC1 and HDAC2.
• Subcellular Location: Nucleus.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin- embedded human lung adenocarcinoma tissue using HDAC1 (Ab-421/423) Antibody.)

Related Product Information for anti-HDAC1 antibody

Entrez Summary: Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]

NCBI and Uniprot Product Information

• NCBI GI #: 13128860
• NCBI GeneID: 3065
• NCBI Accession #: NP_004955.2
• NCBI GenBank Nucleotide #: NM_004964.2
• UniProt Accession #: Q13547; Q92534
• Molecular Weight: 55
• NCBI Official Full Name: histone deacetylase 1
• NCBI Official Synonym Full Names: histone deacetylase 1
• NCBI Official Symbol: HDAC1
• NCBI Official Synonym Symbols: HD1; RPD3; GON-10; RPD3L1
• NCBI Protein Information: histone deacetylase 1
• UniProt Protein Name: Histone deacetylase 1
• UniProt Gene Name: HDAC1
• UniProt Synonym Gene Names: RPD3L1; HD1

NCBI Description

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC1: a transcriptional regulator of the histone deacetylase family, subfamily 1. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events.

Protein type: EC 3.5.1.98; Hydrolase; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1p35.2-p35.1

Cellular Component: chromatin; cytoplasm; cytosol; histone deacetylase complex; nuclear chromatin; nucleoplasm; nucleus; NuRD complex; protein complex; Sin3 complex

Molecular Function: deacetylase activity; enzyme binding; histone deacetylase activity; histone deacetylase binding; NF-kappaB binding; nucleosomal DNA binding; protein binding; protein deacetylase activity; protein N-terminus binding; transcription activator binding; transcription factor activity; transcription factor binding

Biological Process: ATP-dependent chromatin remodeling; blood coagulation; chromatin remodeling; circadian regulation of gene expression; embryonic digit morphogenesis; epidermal cell differentiation; establishment and/or maintenance of chromatin architecture; histone deacetylation; methylation-dependent chromatin silencing; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; odontogenesis of dentine-containing teeth; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid deacetylation